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The effect of zinc(II) on the growth of E . coli studied by microcalorimetry

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Abstract

By using an LKB2277 Bioactivity Monitor, stop-flow mode, the power-time curves of E . coli at 37°C effected by zinc(II) were determined. Some parameters, such as growth rate constants k, inhibitory ratio I, the maximum heat production rate P max heat output Q and the time in the maximum heat production t max were obtained. According these parameters, we found that a low concentration of zinc(II) had a promoting action on the growth of E. coli, but a high concentration of zinc(II) had an inhibitory action. The toxicity of zinc(II) can also be expressed as half inhibitory concentration IC 50 of zinc(II), i.e., 50% effective in this inhibition. The value of IC 50 of zinc(II) on E. coliis 28.09 µg mL-1. The assay is quantitative, inexpensive and versatile.

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References

  1. K. Wang, Trace Elements in Life Sciences, Chinese Computation Science Press, (1996) 208.

  2. J. S. Bond, D. O. Hall and S. Hawkins, J. Bio. Chem., 258 (1983) 8004.

    Google Scholar 

  3. Y. Liu, X. Q. Wang, C. L. Xie and S. S. Qu, Acta Physico-Chimica Sinica, 12 (1996) 659.

    Google Scholar 

  4. J. Yao, Y. Liu, Z. T. Gao, P. Liu, M. Sun, S. S. Qu and Z. N. Yu, J. Therm. Anal. Cal., 70 (2002) 415.

    Article  Google Scholar 

  5. Y. Liu, T. T. Than, P. Shen and S. S. Qu, J. Biochem. Biophys., 40 (2000) 845.

    Google Scholar 

  6. K. Györyová, E. Szunyogová, J. Kovárcaronová D. Hudecová, D. Mudroncaronová and E. Juhászová, J. Anal. Cal., 72 (2003) 587.

    Article  Google Scholar 

  7. M. S. Mcguiness and B. Georges Barisas, Environ. Sci. Technol., 25 (1991) 1092.

    Google Scholar 

  8. I. Lamprecht and B. Schaarschmidt, Application of Calorimetry in Life Sciences. de Gruyter & Co. Berlin 1977.

  9. R. J. Miles, A. E. Beezer and D. H. Lee, Microbios, 45 (1986) 7.

    Google Scholar 

  10. J. Suurkuusk and I. Wasdö, Chem. Scr, 20 (1982)155.

    Google Scholar 

  11. Z. Ali, W. T. O’Hare and B. J. Theaker, J. Therm. Anal. Cal., 71 (2003) 155.

    Article  Google Scholar 

  12. Y. Yang, Y. Liu, J. Zhu and P. Shen, J. Therm. Anal. Cal., 75 (2004) 293.

    Article  Google Scholar 

  13. J. Wang, C. C. Chun and S. Tao, Acta Ecologica Sinica, 12 (1992) 201.

    Google Scholar 

  14. S. M. Mcguiness, D. A. Roess and B. G. Barisas, Thermochim. Acta, 72 (1990)131.

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. School of Environmental Studies, China University of Geosciences, Wuhan, Hubei, 430074, P. R. China

    J. Yao & C. Zhang

  2. Department of Chemistry, College of Chemistry and Molecular Science, Wuhan University, Wuhan, Hubei, 430072, P. R. China

    J. Yao, Y. Liu, H. G. Liang, J. Z. Zhu, X. Qin & S. S. Qu

  3. Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry, Huazhong Agricultural University, Wuhan, Hubei, 430070, P. R. China

    J. Yao, M. Sun & Z. N. Yu

Authors
  1. J. Yao
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  2. Y. Liu
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  3. H. G. Liang
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  4. C. Zhang
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  5. J. Z. Zhu
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  6. X. Qin
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  7. M. Sun
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  8. S. S. Qu
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  9. Z. N. Yu
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Correspondence to J. Yao.

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Yao, J., Liu, Y., Liang, H.G. et al. The effect of zinc(II) on the growth of E . coli studied by microcalorimetry. J Therm Anal Calorim 79, 39–43 (2005). https://doi.org/10.1007/s10973-004-0559-4

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  • DOI: https://doi.org/10.1007/s10973-004-0559-4

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