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Green Synthesis of Ag Nanoparticles with Anti-bacterial Activity Using the Leaf Extract of an African Medicinal Plant, Ipomoea asarifolia (Convolvulaceae)

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Abstract

Herbal drugs prepared with the plant Ipomoea asarifolia has many medicinal uses throughout West Africa, including its employ as diuretic, emmenagogue and purgative. This species is a good source of acylated anthocyanins, and the methanol and ethyl acetate extracts of I. asarifolia are known for their strong acetylcholinesterase inhibitory effects. Here, green synthesis of Ag nanoparticles (Ag NPs) was performed at room temperature using the I. asarifolia leaf extract. The Ag NPs were characterized by UV–visible spectroscopy, FT-IR, XRD, AFM, SEM with EDX and TEM. AFM and SEM analyses showed that spherical morphology of I. asarifolia-synthesized Ag NPs with a size range of 20 to 60 nm. TEM revealed that I. asarifolia-synthesized Ag NPs had a mean particle size of 40 nm. The I. asarifolia-synthesized Ag NPs was tested against four bacterial pathogens using the disk diffusion method. The zone of inhibition varied  significantly in a dose-dependent manner. Overall, our results underlined the potential of cheap aqueous extracts from this African medicinal plant species for the synthesis of silver nanocomplexes with wide efficacy against human pathogenic bacteria.

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Acknowledgements

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through research Group No RG-1438-074.

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Authors and Affiliations

  1. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Jamal M. Khaled, Naiyf S. Alharbi, Shine Kadaikunnan, Ahmed S. Alobaidi & Mohammed N. Al-Anbr

  2. Department of Botany, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India

    Kasi Gopinath & Ayyakannu Aurmugam

  3. Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India

    Marimuthu Govindarajan

  4. Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124, Pisa, Italy

    Giovanni Benelli

  5. The BioRobotics Institute, Scuola Superiore Sant’Anna, Viale Rinaldo Piaggio 34, 56025, Pontedera, Pisa, Italy

    Giovanni Benelli

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  1. Jamal M. Khaled
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  2. Naiyf S. Alharbi
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Correspondence to Shine Kadaikunnan or Kasi Gopinath.

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Khaled, J.M., Alharbi, N.S., Kadaikunnan, S. et al. Green Synthesis of Ag Nanoparticles with Anti-bacterial Activity Using the Leaf Extract of an African Medicinal Plant, Ipomoea asarifolia (Convolvulaceae). J Clust Sci 28, 3009–3019 (2017). https://doi.org/10.1007/s10876-017-1271-4

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