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Mosaic embryo transfer after oocyte in vitro maturation in combination with non-invasive prenatal testing (NIPT)—first report of a euploid live birth

  • Assisted Reproduction Technologies
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Abstract

Purposes

The purpose of this study is to describe a healthy life birth after a mosaic embryo transfer in oocyte in vitro maturation (IVM).

Methods

Patient received minimal stimulation, starting on day 3 after menstrual period. No hCG trigger was administered. Oocyte retrieval was performed and oocytes were matured for 30 h. After denuding, mature oocytes were inseminated by ICSI. Embryos were cultured until blastocyst stage and biopsied.

Results

One euploid embryo after array comprehensive genome hybridization (aCGH) was diagnostic. However, the next-generation sequencing (NGS) re-analysis showed that embryo was a mosaic for chromosome 13 and 21. Nevertheless, pregnancy ultrasound scans and non-invasive prenatal test (NIPT–Verifi-Illumina) indicated a normal fetus development. Finally, a healthy baby was born after 38 weeks. Its weight was 4480 g, head circumference 36 cm, and total length of 51 cm. To confirm that the baby was chromosomically normal, an NGS test was performed in buccal cells, a normal profile was obtained.

Conclusions

Our finding confirmed that mosaic embryo transfer would bring a healthy offspring.

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Correspondence to Luis Guzmán.

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Inoue, N., Lopez, R., Delgado, A. et al. Mosaic embryo transfer after oocyte in vitro maturation in combination with non-invasive prenatal testing (NIPT)—first report of a euploid live birth. J Assist Reprod Genet 34, 1199–1205 (2017). https://doi.org/10.1007/s10815-017-0974-7

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  • DOI: https://doi.org/10.1007/s10815-017-0974-7

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