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Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes

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Abstract

Purpose

By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocyst culture.

Methods

After 1PN zygotes are sequentially cultured for 5 days, the blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in situ hybridization (FISH) with probes for chromosomes 18, X and Y; G-banding analysis was adopted to analyze the karyotype of 1PN hESCs.

Results

The diploid rate of blastocysts was 74.6%, which was significantly (P < 0.001) higher than that of arrested cleavage-stage embryos (31.6%), and the diploid rate of hESCs was 97.0%, which was significantly (P < 0.01) higher than that of blastocysts; the haploid embryos were excluded by blastocyst culture; nevertheless, there still existed such chromosomal abnormalities as mosaic and monosomic in blastocysts and trisomy in hESCs.

Conclusions

Blastocyst culture is an effective method to select against chromosomal abnormalities, especially the haploids in 1PN embryos; however, development to the blastocyst stage is not a reliable marker for mosaicism or aneuploidy.

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Acknowledgement

This work was supported by the Ministry of Science and Technology of China (863 program 2006AA02A102), the National Basic Research Program of China (973 program 2007CB948103).

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Authors and Affiliations

  1. Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, Hunan, China

    Hongqing Liao, Qi OuYang, Ge Lin, Yifan Gu, Changfu Lu, Fei Gong & Guangxiu Lu

  2. CITIC-Xiangya Reproductive and Genetic Hospital, Changsha, Hunan, China

    Hongqing Liao, Shuoping Zhang, Dehua Cheng, Ge Lin, Yifan Gu, Changfu Lu, Fei Gong & Guangxiu Lu

  3. National Engineering and Research Center of Human Stem Cell, 88th Xiangya Road, Changsha, Hunan, China

    Qi OuYang, Ge Lin & Guangxiu Lu

Authors
  1. Hongqing Liao
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  2. Shuoping Zhang
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  3. Dehua Cheng
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  4. Qi OuYang
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  5. Ge Lin
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  6. Yifan Gu
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  7. Changfu Lu
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  8. Fei Gong
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  9. Guangxiu Lu
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Corresponding author

Correspondence to Guangxiu Lu.

Additional information

Capsule

Blastocyst culture is an effective method to select against the haploid in 1PN embryos, though not against the mosaic or aneuploid.

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Liao, H., Zhang, S., Cheng, D. et al. Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes. J Assist Reprod Genet 26, 583–589 (2009). https://doi.org/10.1007/s10815-009-9355-1

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  • DOI: https://doi.org/10.1007/s10815-009-9355-1

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