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Study of antioxidant activities of sulfated polysaccharides from Laminaria japonica

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Abstract

The composition, molecular weight and in vitro antioxidant activity of various sulfated polysaccharides obtained by anion exchange chromatography, acid hydrolysis and radical process degradation of the crude sulfated polysaccharide extracted from Laminaria japonica were compared. The low sulfated F-A2, with a peak-molecular weight (Mp) of 5–15 kDa, 14.5% sulfated ester and 21.8% glucuronic acid, exhibited a very strong antioxidant activity on superoxide and hydroxyl radicals, with activity even higher than that of large molecular weight fractions F-A and F-B. However, highly sulfated fractions with a peak-molecular weight below 15 kDa had much lower antioxidant activities than other fractions. These results indicated that the sulfate group of the low molecular weight fractions represents a physical block for the reaction with oxygen radicals. The chemical properties and antioxidant activities of sulfated polysaccharide fractions obtained by radical process degradation of crude sulfated polysaccharide were quite different from those obtained by acid hydrolysates. By radical process degradation, the high molecular weight was decreased to give LM2 (Mp 8 kDa) and LM1 (Mp 1.5 kDa), with a yield of 40% and 15%, respectively. LM2 was enriched with fucose and sulfated ester, while containing low amounts of glucuronic acid. The antioxidant activity showed that LM2 was unable to scavenge either superoxide or hydroxyl radical, which suggested that radical process degradation targeted mainly ascopyllan-like species rich in glucuronic acid, while the fraction rich in sulfated l-fucose remained unchanged. However, LM1 with Mp 1.5 kDa still retained apparent scavenging ability for superoxide radical, although it contained no glucuronic acid and certain amounts of galactose and mannose as main neutral sugars. These result suggest that the antioxidant activity of sulfated polysaccharides is apparently related not only to molecular weight and sulfated ester content, as previously determined, but also to glucuronic acid and fucose content.

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Acknowledgments

This work was supported in part by projects 2001AA625030 and 2003AA625030of HI-Tech Research and Development Program of China.

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Authors and Affiliations

  1. College of Food Science and Technology, Ocean University of China, No.5, Yu Shan Road, Qingdao, Shandong Province, 266003, People’s Republic of China

    Xue Zhao, Chang-Hu Xue & Ba-Fang Li

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  1. Xue Zhao
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  2. Chang-Hu Xue
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  3. Ba-Fang Li
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Correspondence to Chang-Hu Xue.

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Zhao, X., Xue, CH. & Li, BF. Study of antioxidant activities of sulfated polysaccharides from Laminaria japonica . J Appl Phycol 20, 431–436 (2008). https://doi.org/10.1007/s10811-007-9282-4

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  • DOI: https://doi.org/10.1007/s10811-007-9282-4

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