Abstract
The effects of light and nitrogen deficiency on biomass, fatty acid content and composition were studied in Parietochloris incisa, the unicellular freshwater chlorophyte accumulating very high amounts of arachidonic-acid-rich triacylglycerols. P. incisa cultures grown on complete nutrient medium and under high light (400 μmol photons m− 2 s−1) showed the highest rate of growth in comparison to medium (200 μmol photons m−2 s−1) and low (35 μmol photons m−2 s−1) light intensity. Cultures grown under high light (on complete BG-11 medium) attained higher volumetric contents of total fatty acids and arachidonic acid due to greater increase in biomass. Nitrogen starvation brought about a strong increase in the arachidonic acid proportion of total fatty acids. Thus, adjustments to cultivation conditions could serve as an efficient tool for manipulation of yield and relative content of arachidonic acid in P. incisa. The significance of the changes in lipid metabolism for adaptation of P. incisa to high-light stress and nitrogen deficiency is also discussed.
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Abbreviations
- AA:
-
Arachidonic acid
- DW:
-
Dry weight
- FA:
-
Fatty acids
- PFD:
-
Photon Flux Density
- TAG:
-
Triacylglycerols
- TFA:
-
Total fatty acids
- PUFA:
-
Polyunsaturated fatty acids
References
Ackman RG (1969) Gas-liquid chromatography of fatty acids and esters. In: Lowenstein JM (ed) Methods in Enzymology, vol 14. Academic, New York, pp 329–381
Asada K (1994) Production and action of active oxygen species in photosynthetic tissues. In: Foyer CH, Mullineaux PM (eds) Causes of photooxidative stress and amelioration of defense systems in plants. CRC, Boca Raton, pp 77–104
Bigogno C, Khozin-Goldberg I, Adlerstein D, Cohen Z (2002a) Biosynthesis of arachidonic acid in the oleaginous microalga Parietochloris incisa (Chloropyceae): radiolabeling studies. Lipids 37:209–216
Bigogno C, Khozin-Goldberg I, Boussiba S, Vonshak A, Cohen Z (2002b) Lipid and fatty acid composition of the green oleaginous alga Parietochloris incisa, the richest plant source of arachidonic acid. Phytochemistry 60:497–503
Cheng-Wu Z, Cohen Z, Khozin-Goldberg I, Richmond A (2002) Characterization of growth and arachidonic acid production of Parietochloris incisa comb. nov (Trebouxiophyceae, Chlorophyta). J Appl Phycol 14:453–460
Cohen Z (1986) Products from microalgae. In: Richmond A (ed) Handbook for microalgal mass culture. CRC, Boca Raton, pp 421–454
Cohen Z (1999) Production of polyunsaturated fatty acids by the microalga Porphyridium cruentum. In: Cohen Z (ed) Production of chemicals by microalgae. Taylor and Francis, London, pp 1–24
Cohen Z, Vonshak A, Richmond A (1988) Effect of environmental conditions on fatty acid composition of the red alga Porphyridium cruentum: correlation to growth rate. J Phycol 24:328–332
Cohen Z, Norman HA, Heimer YM (1993) Potential use of substituted pyridazinones for selecting polyunsaturated fatty acid overproducing cell lines of algae. Phytochemistry 32:259–264
Gombos Z, Kanervo E, Tsvetkova N, Sakamoto T, Aro E M, Murata N (1998) Genetic enhancement of the ability to tolerate photoinhibition by introduction of unsaturated bonds into membrane glycerolipids. Plant Physiol 115:551–559
Hansen J, Schade D, Harris C, Merkel K, Adamkin D, Hall R, Lim M, Moya F, Stevens D, Twist P (1997) Docosahexaenoic acid plus arachidonic acid enhance preterm infant growth. Prostaglandins, Leukotriens, Essential Fatty Acids 57:157
Henderson RJ, Sargent JR (1989) Lipid composition and biosynthesis in aging cultures of the marine cryptomonad Chroomonas salina. Phytochemistry 28:1355–1362
Khozin I, Adlerstein D, Bigogno C, Heimer YM, Cohen Z (1997) Elucidation of the biosynthesis of eicosapentaenoic acid in the microalga Porphyridium cruentum. Studies with radiolabeled precursors. Plant Physiol 114:223–230
Khozin-Goldberg I, Hu ZY, Adlerstein D, Didi Cohen S, Heimer YM, Cohen Z (2000) Triacylglycerols of the red microalga Porphyridium cruentum participate in the biosynthesis of eukaryotic galactolipids. Lipids 5:881–889
Khozin-Goldberg I, Bigogno C, Shreshta P, Cohen Z (2002) Nitrogen starvation induces the accumulation of arachidonic acid in the freshwater green alga Parietochloris incisa (Trebouxiophyceae). J Phycol 38:991–994
Klyachko-Gurvich G, Tsoglin LN, Doucha J, Kopetskii J, Shebalina BI, Semenenko VE (1999) Desaturation of fatty acids as an adaptive response to shifts in light intensity. Physiol Plant 107:240–249
Koletzko B, Braun M (1991) Arachidonic acid and early human growth: is there a relation? Ann Nutr Metabol 35:128–131
Lichtenthaler HK (1987) Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. In: Lowenstein JM (ed) Methods in Enzymology, vol 14. Academic, New York, pp 331–382
Mendoza H, Martel A, Jimenez del Rio M, Garcia Reina G (1999) Oleic acid is the main fatty acid related with carotenogenesis in Dunaliella salina. J Appl Phycol 11:15–19
Merzlyak MN, Chivkunova OB, Gorelova OA, Reshetnikova IV, Solovchenko AE, Khozin-Goldberg I, Cohen Z (2007) Effect of nitrogen starvation on optical properties, pigments and arachidonic acid content of the unicellular green alga Parietochloris incisa Trebouxiophyceae, Chlorophyta). J Phycol 43:833–843
Molina Grima E, Garcia Camacho F, Acien Fernandez FG (1999) Production of EPA from Phaeodactylum tricornutum. In: Cohen Z (ed) Chemicals from microalgae. Taylor and Francis, London, pp 57–92
Niyogi K (1999) Photoprotection revisited: genetic and molecular approaches. Annu Rev Plant Physiol Mol Biol 50:333–359
Piorreck M, Baasch KH, Pohl P (1984) Biomass production, total protein, chlorophyll, lipids and fatty acids of freshwater green and blue algae under different nitrogen regimes. Phytochemistry 23:207–216
Rabbani S, Beyer P, Lintig J, Hugueney P, Kleinig H (1998) Induced β-carotene synthesis driven by triacylglycerol deposition in the unicellular alga Dunaliella bardawii. Plant Physiol 116:1239–1248
Rodrigues MA, dos Santos CP, Young AJ, Strbac D, Hall DO (2002) A smaller and impaired xanthophyll cycle makes the deep sea macroalgae Laminaria abyssalis (phaeophyceae) highly sensitive to daylight when compared with shallow water Laminaria digitata. J Phycol 38:939–947
Shifrin NS, Chishlom SW (1981) Phytoplankton lipids: interspecific differences and effects of nitrate, silicate, and light–dark cycles. J Phycol 17:374–384
Stanier RY, Kunisawa MM, Cohen-Bazir G (1971) Purification and properties of unicellular blue-green algae (order Chlorococcales). Bacteriol Rev 35:171–201
Sukenik A (1999) Production of eicosapentaenoic acid by the marine eustigmatophyte Nannochloropsis. In: Cohen Z (ed) Chemicals from microalgae. Taylor and Francis, London, pp 41–56
Thompson GA Jr (1996) Lipids and membrane function in green algae. Biochim Biophys Acta 1302:17–45
Watanabe S, Hirabashi S, Boussiba S, Cohen Z, Vonshak A, Richmond A (1996) Parietochloris incisa comb. Nov. (Trebouxiophyceae, Chlorophyta). Phycol Res 44:107–108
Acknowledgements
This work was supported in part by fellowships from the BIDR to M.N.M. and A.E.S. M.N.M. and A.E.S. also acknowledge the financial support of the Russian Fund for Basic Research (Grant # 06-04-48883) and Russia President’s Grant Council (Ministry of Science of the Russian Federation).
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Solovchenko, A.E., Khozin-Goldberg, I., Didi-Cohen, S. et al. Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa . J Appl Phycol 20, 245–251 (2008). https://doi.org/10.1007/s10811-007-9233-0
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DOI: https://doi.org/10.1007/s10811-007-9233-0