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MicroRNA let-7c Improves LPS-Induced Outcomes of Endometritis by Suppressing NF-κB Signaling

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Abstract

Endometritis is a common inflammatory disease which endangers human and animal reproductive health. MicroRNA (miRNA) let-7c plays an important role in the inflammatory process; however, the regulatory underlying mechanism of let-7c in endometritis is unclear. In this study, we confirmed that let-7c was significantly reduced in LPS-induced mouse endometritis model, and overexpression of let-7c was able to effectively reduce uterine tissue damage caused by lipopolysaccharide (LPS), and then, a LPS-induced bovine endometrial epithelial cell (BEND) line was used to mimic the inflammatory model in vitro. Our data showed that overexpression of let-7c significantly reduced the expression of pro-inflammatory cytokines in BEND cells induced by LPS. Meanwhile, immunofluorescence and western blotting results showed that let-7c significantly inhibited LPS-induced phosphorylation of NF-κB, thereby inhibiting downstream pro-inflammatory cytokine expression. Taken together, our results suggested that let-7c ameliorates LPS-induced endometritis by attenuating the expression of pro-inflammatory cytokines via inhibition of the activation of NF-κB.

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Acknowledgements

Thank all members in the Laboratory of Veterinary Clinical Diagnosis with all the helpful suggestions. We especially thank Dr. Aftab Shaukat for English language editing.

Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31772816).

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Authors and Affiliations

  1. Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Gan Zhao, Tao Zhang, Haichong Wu, Kangfeng Jiang, Changwei Qiu & Ganzhen Deng

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  1. Gan Zhao
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  2. Tao Zhang
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  3. Haichong Wu
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  4. Kangfeng Jiang
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  5. Changwei Qiu
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Correspondence to Ganzhen Deng.

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The authors declare that they have no conflict of interest.

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Zhao, G., Zhang, T., Wu, H. et al. MicroRNA let-7c Improves LPS-Induced Outcomes of Endometritis by Suppressing NF-κB Signaling. Inflammation 42, 650–657 (2019). https://doi.org/10.1007/s10753-018-0922-4

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