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Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation

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Abstract

The effect of methyl jasmonate (MeJA) treatment on diploid and tetraploid plants of Matricaria chamomilla and the changes of the main physiological parameters and secondary metabolites content was studied. Leaf rosettes from 7-week-old plants were harvested at four different time points (24, 48, 72, 96 h) after foliar application of 0.4 mM MeJA. The treatment led to a moderate biomass accumulation accompanied by an accumulation of photosynthetic pigments and decrease in the total soluble proteins. The content of hydrogen peroxide and superoxide radical was most elevated at 24 and 72 h after the treatment. Although the values of stress parameters were higher in tetraploid plants (such as in control so in treated plants), their total increase after the MeJA application was similar. The absolute level of total soluble phenols and flavonoids in the controls was similar in both cultivars and their content was enhanced in 24- and 48-h variants, more rapidly in diploid plants. MeJA has been found to trigger different responses of the secondary metabolites accumulation. Almost all studied metabolites were higher in control plants in the tetraploid cultivar, but their biosynthesis was more stimulated in diploids. Significant changes, mainly increasing trends with a maximum between 24 and 48 h after elicitation, in the content of free coumarin herniarin and its glycosidic precursors (Z)- and (E)-2-β-d-glucopyranosyloxy-4-methoxycinnamic acid were observed. The umbelliferone level increased over time. The maximum values for chlorogenic acid and 1,5-dicaffeoylquinic acid were at 24-h and between the 24–72 h time points. Among dicycloethers, the amounts of (E)-form decreased with time, and for (Z)-form an opposite trend was observed, with a maximum of 96 h for diploid and 24 h for tetraploid after elicitation.

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Abbreviations

DW:

Dry weight

GMCA:

(Z)- and (E)-2-β-d-glucopyranosyloxy-4-methoxycinnamic acid

MeJA:

Methyl jasmonate

PAL:

Phenylalanine ammonia-lyase

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Acknowledgments

This study was financially supported by the Slovak Grand Agency under contract VEGA 1/0046/14 and the UPJS internal grant system under contract VVGS PF-2013-95. We thank Mrs. Anna Michalčová and Mrs. Margita Buzinkaiová for their valuable technical assistance.

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, Institute of Biology and Ecology, P. J. Šafárik University, Mánesova 23, 041 54, Košice, Slovakia

    Zuzana Dučaiová, Matúš Sajko, Silvia Mihaličová & Miroslav Repčák

  2. Department of Biology, Faculty of Science, University of Hradec Králové, Rokitanského 62, 500 03, Hradec Králové, Czech Republic

    Zuzana Dučaiová

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  1. Zuzana Dučaiová
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  2. Matúš Sajko
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Dučaiová, Z., Sajko, M., Mihaličová, S. et al. Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation. Plant Growth Regul 79, 81–94 (2016). https://doi.org/10.1007/s10725-015-0114-2

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