Abstract
In order to understand early molecular events associated with increase in fruit size and woolliness prevention induced by pre-harvest gibberellic acid (GA3) spraying, differential transcript accumulation of genes encoding proteins putatively involved in protein folding and protection, cell wall metabolism, and endomembrane transport was studied during fruit development of ‘Chiripá’ peach. Woolliness occurrence reached 100% in untreated peach, was reduced by 15% with GA3 spraying at the end of the pit hardening stage and was significantly reduced (by 78%) in peaches treated at the beginning of the pit hardening stage despite a significantly increased fruit size. Low incidence of woolliness after cold storage and fruit size increase in early GA3 treated peach was correlated with high transcript accumulation of genes encoding proteins putatively involved in protein folding, and protection of the endoplasmic reticulum (heat shock proteins—HSP40 er) and chloroplast (HSP17.8 ch), endomembrane transport (GTPase), as well as genes involved in cell wall loosening (expansins—Exp1, Exp2, Exp3, Exp4).
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Abbal P, Pradal M, Muniz L, Sauvage F-X, Chatelet P, Ueda T, Tesniere C (2008) Molecular characterization and expression analysis of the Rab GTPase family in Vitis vinifera reveal the specific expression of a VvRabA protein. J Exp Bot 59:2403–2416
Amarante CVT, Drehmer AMF, Souza F, Francescato P (2005) Preharvest spraying with gibberellic acid (GA3) and aminoethoxyvinilglycine (AVG) delays fruit maturity and reduces fruit losses on peaches. Revista Brasileira de Fruticultura 27:1–5
Arnaud N, Girin T, Sorefan K, Fuentes S, Wood TA, Lawrenson T, Sablowski R, Ostergaard L (2010) Gibberellins control fruit patterning in Arabidopsis thaliana. Genes Dev 24:2127–2132
Ben-Arie R, Saks Y, Sonego L, Frank A (1996) Cell wall metabolism in gibberellin-treated persimmon fruits. Plant Growth Regul 19:25–33
Brummell DA, Harpster MH, Dunsmuir P (1999) Differential expression of expansin gene family members during growth and ripening of tomato fruit. Plant Mol Biol 39:161–169
Brummell DA, Dal Cin V, Lurie S, Crisosto CH, Labavitch JM (2004) Cell wall metabolism during the development of chilling injury in cold-stored peach fruit: association of mealiness with arrested disassembly of cell wall pectins. J Exp Bot 55:2041–2052
Choi D, Cho H-T, Lee Y (2006) Expansins: expanding importance in plant growth and development. Physiol Plant 126:511–518
Ferri VC, Rinaldi MM, Silva JA, Luchetta L, Marini L, Rombaldi CV (2004) Gibberellic acid on ripening delay of Kakis (Diospyrus kaki L.) cultivar Fuyu. Ciência e Tecnologia de Alimentos 24:1–5
Gallego-Bartolomé J, Minguet EG, Marín JA, Prat S, Blázquez MA, Alabadí D (2010) Transcriptional diversification and functional conservation between DELLA proteins in Arabidopsis. Mol Biol Evol 27:1247–1256
Girardi CL, Corrent AR, Lucchetta L, Zanuzo MR, Da Costa TS, Brackmann A, Twyman RM, Nora FR, Nora L, Silva JA, Rombaldi CV (2005) Effect of ethylene, intermittent warming and controlled atmosphere on postharvest quality and the occurrence of woolliness in peach (Prunus persica cv. Chiripa) during cold storage. Postharvest Biol Technol 38:25–33
González-Agüero M, Pavez L, Ibánez F, Pacheco I, Campos-Vargas R, Meisel LA, Orellana A, Retamales J, Silva H, González M, Cambiazo V (2008) Identification of woolliness response genes in peach fruit after post-harvest treatments. J Exp Bot 59:1973–1986
Harrison EP, McQueen-Mason SJ, Manning K (2001) Expression of six expansin genes in relation to extension activity in developing strawberry fruit. J Exp Bot 52:1437–1446
Hittinger CT, Carroll SB (2007) Gene duplication and the adaptive evolution of a classic genetic switch. Nature 449:677–681
Hiwasa K, Rose JKC, Nakano R, Inaba A, Kubo Y (2003) Differential expression of seven α-expansin genes during growth and ripening of pear fruit. Physiol Plantarum 117:564–572
Hu J, Mitchum MG, Barnaby N, Ayele BT, Ogawa M, Nam E, Lai W-C, Hanada A, Alonso JM, Ecker JR, Swain SM, Yamaguchi S, Kamiya Y, Sun T-P (2008) Potential sites of bioactive gibberellin production during reproductive growth in Arabidopsis. Plant Cell 20:320–336
Jackson DI (1968) Gibberellin and the growth of peach and apricot fruits. Aust J Biol Sci 21:209–216
Ju Z, Duan Y, Ju Z (1999) Combinations of GA(3) and AVG delay fruit maturation, increase fruit size and improve storage life of ‘Feicheng’ peaches. J Hort Sci Biotechnol 74:579–583
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−∆∆CT method. Methods 25:402–408
Lurie S, Crisosto CH (2005) Chilling injury in peach and nectarine. Postharvest Biol Technol 37:195–208
Martínez-Romero D, Valero D, Serrano M, Burlo F, Carbonell A, Burgos L, Riquelme F (2000) Exogenous polyamines and gibberellic acid effects on peach (Prunus persica L.) storability improvement. J Food Sci 65:288–294
Obenland DM, Crisosto CH, Rose JKC (2003) Expansin protein levels decline with the development of mealiness in peaches. Postharvest Biol Technol 29:11–18
Ozga J, Dennis M (2003) Hormonal interactions in fruit development. J Plant Growth Regul 22:73–81
Pegoraro C, Manica-Berto R, Chaves FC, Borges CT, Franco JJ, Rombaldi CV, Silva JA (2010a) Differential expression of genes associated with endomembrane transport and cell wall metabolism during ripening of ‘Chimarrita’ and ‘Granada’ peach. Hort Environ Biotechnol 51:207–211
Pegoraro C, Zanuzo MR, Chaves FC, Brackmann A, Girardi CL, Lucchetta L, Nora L, Silva JA, Rombaldi CV (2010b) Physiological and molecular changes associated with prevention of woolliness in peach following pre-harvest application of gibberellic acid. Postharvest Biol Technol 57:19–26
Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N, Brainsted J, Klapa M, Currier T, Thiagarajan M, Sturn A, Snuffin M, Rezantsev A, Popov D, Ryltsov A, Kostukovich E, Borisovski I, Liu Z, Vinsavichi A, Trush V, Quackenbusch J (2003) TM4: a free, open-source system for microarray data management and analysis. Biotechniques 34:374–378
Sun J-H, Chen J-Y, Kuang J-F, Chen W-X, Lu W-J (2010) Expression of sHSP genes was affected by heat shock and cold acclimation in relation to chilling tolerance in plum fruit. Postharvest Biol Technol 55:91–96
Thomas SG, Rieu I, Steber CM (2005) Gibberellin metabolism and signaling. Vitam Horm 7:289–338
Trainotti L, Zanin D, Casadoro G (2003) A cell wall-oriented genomic approach reveals a new and unexpected complexity of the softening in peaches. J Exp Bot 54:1821–1832
Trainotti L, Bonghi C, Ziliotto F, Zanin D, Rasori A, Casadoro G, Ramina A, Tonutti P (2006) The use of microarray μPEACH1.0 to investigate transcriptome changes during transition from pre-climacteric to climacteric phase in peach fruit. Plant Sci 170:606–613
Vriesen WH, Feron R, Maretto F, Keijman J, Mariani C (2008) Changes in tomato ovary transcriptome demonstrate complex hormonal regulation of fruit set. New Phytol 177:60–76
Xie H, Chen J, Yuan R, Zhong Y, Feng H, Xu S, Li J, Wang L (2009) Differential expression and regulation of expansin gene family members during fruit growth and development of ‘Shija’ longan fruit. Plant Growth Regul 58:225–233
Zhang C, Lee U, Tanabe K (2008) Hormonal regulation of fruit set, parthenogenesis induction and fruit expansion in Japanese pear. Plant Growth Regul 55:231–240
Zilkah S, Lurie S, Lapsker Z, Zuthi Y, David I, Yesselson Y, Antman S, Ben-Arie R (1997) The ripening and storage quality of nectarine fruits in response to preharvest application of gibberellic acid. J Hort Sci Biotechnol 72:355–362
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To Professor Antônio Costa Oliveira for his help with qPCR analyses and to CAPES and CNPq for financial support.
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Pegoraro, C., Chaves, F.C., Dal Cero, J. et al. Effects of pre-harvest gibberellic acid spraying on gene transcript accumulation during peach fruit development. Plant Growth Regul 65, 231–237 (2011). https://doi.org/10.1007/s10725-011-9591-0
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DOI: https://doi.org/10.1007/s10725-011-9591-0