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Identification of major QTLs associated with stable resistance of tomato cultivar ‘Hawaii 7996’ to Ralstonia solanacearum

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Abstract

Bacterial wilt caused by Ralstonia solanacearum is one of the most devastating diseases of tomato. Tomato cultivar ‘Hawaii 7996’ has been shown to have stable resistance against different strains under different environments. This study aimed to locate quantitative trail loci (QTLs) associated with stable resistance using 188 recombinant inbred lines (RILs) derived from ‘Hawaii 7996’ and ‘West Virginia 700.’ A new linkage map with good genome coverage was developed, mainly using simple sequence repeat markers developed from anchored bacterial artificial chromosome or scaffold sequences of tomato. The population was evaluated against phylotype I and phylotype II strains at seedling stage or in the field in Indonesia, Philippines, Taiwan, Thailand, and Reunion. Two major QTLs were identified to be associated with stable resistance. Bwr-12, located in a 2.8-cM interval of chromosome 12, controlled 17.9–56.1 % of total resistance variation. The main function of Bwr-12 was related to suppression of internal multiplication of the pathogen in the stem. This QTL was not associated with resistance against race 3-phylotype II strain. Bwr-6 on chromosome 6 explained 11.5–22.2 % of the phenotypic variation. Its location differed with phenotype datasets and was distributed along a 15.5-cM region. The RILs with the resistance allele from both Bwr-12 and Bwr-6 had the lowest disease incidence, which was significantly lower than the groups with only Bwr-12 or Bwr-6. Our studies confirmed the polygenic nature of resistance to bacterial wilt in tomato, and that stable resistance in ‘Hawaii 7996’ is mainly associated with Bwr-6 and Bwr-12.

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Acknowledgments

The authors would like to thank Ms. Dolores R. Ledesma for conducting statistical analyses and Ms. Chiou-fen Hsu for technical assistance. This study is supported by funding provided by GTZ 81070160: Deutsche Gesellschaft für Internationale Zusammenarbeit GmbH of Germany and COA 98as-1.2.1-s-ag: Council of Agriculture of Taiwan.

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Author notes

  1. Hai Thi Hong Truong

    Present address: Department of Horticulture Science, Faculty of Agronomy, Hue University of Agriculture and Forestry, 102 Phung Hung street, Hue City, Vietnam

  2. Conrado H. Balatero

    Present address: East-West Seed Company, Km. 54 Cagayan Valley Rd, Sampaloc, San Rafael, Bulacan, Philippines

Authors and Affiliations

  1. AVRDC – The World Vegetable Center, 60 Yi-Min Liao, Shanhua, Tainan, 74151, Taiwan

    Jaw-Fen Wang, Fang-I Ho, Hai Thi Hong Truong & Shu-Mei Huang

  2. Institute of Plant Breeding, University of the Philippines Los Baños, College, Los Baños, Laguna, Philippines

    Conrado H. Balatero

  3. Hortigenetics Research Ltd., Chiangmai, Thailand

    Vanla Dittapongpitch

  4. P.T. East-West Seed Indonesia, Purwakarta, Jawa Barat, Indonesia

    Nurul Hidayati

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  1. Jaw-Fen Wang
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Correspondence to Jaw-Fen Wang.

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Wang, JF., Ho, FI., Truong, H.T.H. et al. Identification of major QTLs associated with stable resistance of tomato cultivar ‘Hawaii 7996’ to Ralstonia solanacearum . Euphytica 190, 241–252 (2013). https://doi.org/10.1007/s10681-012-0830-x

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