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Proliferation control strategies to improve productivity and survival during CHO based production culture

A summary of recent methods employed and the effects of proliferation control in product secreting CHO cell lines

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Abstract

Chinese Hamster Ovary cells are the primary system for the production of recombinant proteins for therapeutic use. Protein productivity is directly proportional to viable biomass, viability and culture longevity of the producer cells and a number of approaches have been taken to optimise these parameters. Cell cycle arrest, particularly in G1 phase, typically using reduced temperature cultivation and nutritional control have been used to enhance productivity in production cultures by prolonging the production phase, but the mechanism by which these approaches work is still not fully understood. In this article, we analyse the public literature on proliferation control approaches as they apply to production cell lines with particular reference to what is known about the mechanisms behind each approach.

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Acknowledgements

This work was supported by funding from Science Foundation Ireland (SFI).

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  1. National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, 11, Ireland

    Niraj Kumar, Patrick Gammell & Martin Clynes

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  1. Niraj Kumar
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  2. Patrick Gammell
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  3. Martin Clynes
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Correspondence to Patrick Gammell.

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Kumar, N., Gammell, P. & Clynes, M. Proliferation control strategies to improve productivity and survival during CHO based production culture. Cytotechnology 53, 33–46 (2007). https://doi.org/10.1007/s10616-007-9047-6

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  • DOI: https://doi.org/10.1007/s10616-007-9047-6

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