Skip to main content
SpringerLink
Log in

Antimicrobial activity of silver nanoparticles in situ growth on TEMPO-mediated oxidized bacterial cellulose

  • Original Paper
  • Published:
Cellulose Aims and scope Submit manuscript

Abstract

In order to improve the antimicrobial activity of bacterial cellulose (BC), the silver nanoparticles (Ag NPs) were in situ fabricated on the BC membranes, affording BC and Ag hybrid antimicrobial materials, BC + Ag, which possesses excellent antimicrobial performance. Typically, carboxyl groups were firstly introduced into BC by TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical)-mediated oxidation. Then, the carboxyl-functionalized BC was performed with ion-exchange reaction to change the sodium ions into Ag+ by immersing in AgNO3 aqueous solution, generating Ag+ anchored BC. Finally, two types of distinct reductive reagents including NaBH4 and sodium citrate were employed to transform Ag+ into Ag NPs to fabricate BC + Ag. The diameters of Ag NPs were determined to be 3.8 nm for NaBH4-reduced BC + Ag, and 22.0 nm for sodium citrate-reduced one, respectively. The silver content of BC + Ag were determined to be 1.944 and 2.895 wt% for NaBH4-reduced sample and sodium citrate-reduced one, respectively. Two types of BC + Ag both showed a slow and persistent Ag+ release profile, but the NaBH4-reduced one released much more Ag+ than that of sodium citrate under the same measurement condition. In-depth antibacterial analysis via the disc diffusion and colony forming count method disclosed that BC + Ag exhibited strong bactericidal effects against both Escherichia coli and Staphylococcus aureus. And the antibacterial activity of NaBH4-reduced BC + Ag was higher than the sodium citrate-reduced one. Overall, this study would further improve the antibacterial efficiency of BC + Ag.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

BC:

Bacterial cellulose

TEMPO:

2,2,6,6- tetramethylpiperidine-1-oxyl radical

BC + Ag:

Silver nanoparticles on carboxyl-functionalized BC composites

ROS:

Reactive oxygen species

DP:

Degree of polymerization

E. coli :

Escherichia coli

S. aureus :

Staphylococcus aureus

SH:

Schenk and Hildebrandt medium

SEM:

Scanning electron microscopy

XRD:

X-ray diffraction

ICP-MS:

Inductively coupled plasma mass spectrometry

FTIR:

Fourier transform infrared

TEM:

Transmission electron microscopy

LB:

Luria–Bertani medium

SPR:

Surface plasmon resonance

References

  • Barud HS, Regiani T, Marques RF, Lustri WR, Messaddeq Y, Ribeiro SJ (2011) Antimicrobial bacterial cellulose-silver nanoparticles composite membranes. J Nanomater 2011:10

    Article  Google Scholar 

  • Cao X, Ding B, Yu J, Al-Deyab SS (2013) In situ growth of silver nanoparticles on TEMPO-oxidized jute fibers by microwave heating. Carbohydr Polym 92:571–576

    Article  CAS  Google Scholar 

  • Carr HS, Wlodkowski TJ, Rosenkranz HS (1973) Silver sulfadiazine: in vitro antibacterial activity. Antimicrob Agents Chemother 4:585–587

    Article  CAS  Google Scholar 

  • Chaloupka K, Malam Y, Seifalian AM (2010) Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol 28:580–588

    Article  CAS  Google Scholar 

  • Choi O, Deng KK, Kim N-J, Ross L Jr, Surampalli RY, Hu Z (2008) The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth. Water Res 42:3066–3074

    Article  CAS  Google Scholar 

  • Creighton JA, Blatchford CG, Albrecht MG (1979) Plasma resonance enhancement of Raman scattering by pyridine adsorbed on silver or gold sol particles of size comparable to the excitation wavelength. J Chem Soc Faraday Trans 2 Mol Chem Phys 75:790–798

    Article  CAS  Google Scholar 

  • Czaja W, Krystynowicz A, Bielecki S, Brown RM Jr (2006) Microbial cellulose—the natural power to heal wounds. Biomaterials 27:145–151

    Article  CAS  Google Scholar 

  • Czaja WK, Young DJ, Kawecki M, Brown RM (2007) The future prospects of microbial cellulose in biomedical applications. Biomacromolecules 8:1–12

    Article  CAS  Google Scholar 

  • French AD (2014) Idealized powder diffraction patterns for cellulose polymorphs. Cellulose 21:885–896

    Article  CAS  Google Scholar 

  • He D, Hu B, Yao Q-F, Wang K, Yu S-H (2009) Large-scale synthesis of flexible free-standing SERS substrates with high sensitivity: electrospun PVA nanofibers embedded with controlled alignment of silver nanoparticles. ACS Nano 3:3993–4002

    Article  CAS  Google Scholar 

  • Holladay RJ, Christensen H, Moeller WD (2006) Treatment of humans with colloidal silver composition. US Patents

  • Ifuku S, Tsuji M, Morimoto M, Saimoto H, Yano H (2009) Synthesis of silver nanoparticles templated by TEMPO-mediated oxidized bacterial cellulose nanofibers. Biomacromolecules 10:2714–2717

    Article  CAS  Google Scholar 

  • Isogai A, Saito T, Fukuzumi H (2011a) TEMPO-oxidized cellulose nanofibers. Nanoscale 3:71–85

    CAS  Google Scholar 

  • Isogai T, Saito T, Isogai A (2011b) Wood cellulose nanofibrils prepared by TEMPO electro-mediated oxidation. Cellulose 18:421–431

    Article  CAS  Google Scholar 

  • Kittler S, Greulich C, Diendorf J, Koller M, Epple M (2010) Toxicity of silver nanoparticles increases during storage because of slow dissolution under release of silver ions. Chem Mater 22:4548–4554

    Article  CAS  Google Scholar 

  • Kong H, Jang J (2006) One-step fabrication of silver nanoparticle embedded polymer nanofibers by radical-mediated dispersion polymerization. Chem Commun 28:3010–3012

    Article  Google Scholar 

  • Lee P, Meisel D (1982) Adsorption and surface-enhanced Raman of dyes on silver and gold sols. J Phys Chem 86:3391–3395

    Article  CAS  Google Scholar 

  • Li W-R, Xie X-B, Shi Q-S, Zeng H-Y, Ouyang Y-S, Chen Y-B (2010) Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl Microbiol Biotechnol 85:1115–1122

    Article  CAS  Google Scholar 

  • Li W-R, Xie X-B, Shi Q-S, Duan S-S, Ouyang Y-S, Chen Y-B (2011) Antibacterial effect of silver nanoparticles on Staphylococcus aureus. Biometals 24:135–141

    Article  CAS  Google Scholar 

  • Liu J, Hurt RH (2010) Ion release kinetics and particle persistence in aqueous nano-silver colloids. Environ Sci Technol 44:2169–2175

    Article  CAS  Google Scholar 

  • Lu X, Rycenga M, Skrabalak SE, Wiley B, Xia Y (2009) Chemical synthesis of novel plasmonic nanoparticles. Annu Rev Phys Chem 60:167–192

    Article  CAS  Google Scholar 

  • Maneerung T, Tokura S, Rujiravanit R (2008) Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydr Polym 72:43–51

    Article  CAS  Google Scholar 

  • Marambio-Jones C, Hoek EM (2010) A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanopart Res 12:1531–1551

    Article  CAS  Google Scholar 

  • Martinez-Castanon G, Nino-Martinez N, Martinez-Gutierrez F, Martinez-Mendoza J, Ruiz F (2008) Synthesis and antibacterial activity of silver nanoparticles with different sizes. J Nanopart Res 10:1343–1348

    Article  CAS  Google Scholar 

  • Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16:2346

    Article  CAS  Google Scholar 

  • Nam S, Condon BD (2014) Internally dispersed synthesis of uniform silver nanoparticles via in situ reduction of [Ag (NH3) 2]+ along natural microfibrillar substructures of cotton fiber. Cellulose. doi:10.1007/s10570-014-0270-y

    Google Scholar 

  • Ouyang Y, Xie Y, Tan S, Shi Q, Chen Y (2009) Structure and antibacterial activity of Ce3+ exchanged montmorillonites. J Rare Earths 27:858–863

    Article  Google Scholar 

  • Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73:1712–1720

    Article  CAS  Google Scholar 

  • Panáček A, Kvitek L, Prucek R, Kolar M, Vecerova R, Pizurova N, Sharma VK, Nevečná TJ, Zboril R (2006) Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phys Chem B 110:16248–16253

    Article  Google Scholar 

  • Petersen N, Gatenholm P (2011) Bacterial cellulose-based materials and medical devices: current state and perspectives. Appl Microbiol Biotechnol 91:1277–1286

    Article  CAS  Google Scholar 

  • Saito T, Isogai A (2004) TEMPO-Mediated oxidation of native cellulose. The effect of oxidation conditions on chemical and crystal structures of the water-insoluble fractions. Biomacromolecules 5:1983–1989

    Article  CAS  Google Scholar 

  • Saito T, Shibata I, Isogai A, Suguri N, Sumikawa N (2005) Distribution of carboxylate groups introduced into cotton linters by the TEMPO-mediated oxidation. Carbohydr Polym 61:414–419

    Article  CAS  Google Scholar 

  • Saito T, Okita Y, Nge T, Sugiyama J, Isogai A (2006) TEMPO-mediated oxidation of native cellulose: microscopic analysis of fibrous fractions in the oxidized products. Carbohydr Polym 65:435–440

    Article  CAS  Google Scholar 

  • Saito T, Hirota M, Tamura N, Kimura S, Fukuzumi H, Heux L, Isogai A (2009) Individualization of nano-sized plant cellulose fibrils by direct surface carboxylation using TEMPO catalyst under neutral conditions. Biomacromolecules 10:1992–1996

    Article  CAS  Google Scholar 

  • Saito T, Hirota M, Tamura N, Isogai A (2010) Oxidation of bleached wood pulp by TEMPO/NaClO/NaClO2 system: effect of the oxidation conditions on carboxylate content and degree of polymerization. J Wood Sci 56:227–232

    Article  CAS  Google Scholar 

  • Sanpui P, Murugadoss A, Prasad P, Ghosh SS, Chattopadhyay A (2008) The antibacterial properties of a novel chitosan–Ag-nanoparticle composite. Int J Food Microbiol 124:142–146

    Article  CAS  Google Scholar 

  • Su H-L, Chou C-C, Hung D-J, Lin S-H, Pao I, Lin J-H, Huang F-L, Dong R-X, Lin J-J (2009) The disruption of bacterial membrane integrity through ROS generation induced by nanohybrids of silver and clay. Biomaterials 30:5979–5987

    Article  CAS  Google Scholar 

  • Yang G, Xie J, Hong F, Cao Z, Yang X (2012) Antimicrobial activity of silver nanoparticle impregnated bacterial cellulose membrane: effect of fermentation carbon sources of bacterial cellulose. Carbohydr Polym 87:839–845

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was funded by the Cooperation Projects of Foshan City and Chinese Academy (No. 2012HY100115), Strategic Cooperation Projects Guangdong Province and Chinese Academy (No. 2011B090300018), the Scientific and Technological Project of Guangdong Province (No. 2011B010400039, 2011B070500020) and the Scientific and Technological Project of Guangzhou City (No. 11A24060559).

Author information

Authors and Affiliations

  1. State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, 510070, Guangdong, People’s Republic of China

    Jin Feng, Qingshan Shi, Wenru Li, Xiulin Shu, Aimei Chen, Xiaobao Xie & Xiaomo Huang

Authors
  1. Jin Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qingshan Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wenru Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiulin Shu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Aimei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaobao Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiaomo Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qingshan Shi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Feng, J., Shi, Q., Li, W. et al. Antimicrobial activity of silver nanoparticles in situ growth on TEMPO-mediated oxidized bacterial cellulose. Cellulose 21, 4557–4567 (2014). https://doi.org/10.1007/s10570-014-0449-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10570-014-0449-2

Keywords

Search

Navigation