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In vitro endothelial cell susceptibility to xenobiotics: Comparison of three cell types

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Abstract

In three different endothelial cell (EC) cultures (primary human umbilical cord vein, so-called HUVEC; and immortalized cell lines HBMEC and EA-hy-926), the effects of different xenobiotics were studied in order to standardize vascular EC models for in vitro pharmacotoxicological studies. Cell characteristics were first investigated by the production and the mRNA levels of known endothelial markers in the three EC culture models. EC secretory products, tissue plasminogen activator (tPA) and von Willebrand factor (vWF), were present in the supernatant of the immortalized cell lines. The mRNA levels of vWF, tPA, platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31), and β -integrin subunit, which are involved in the control of platelet function, coagulation, and fibrinolysis as well as in cell–matrix interactions, were investigated in all EC types. For at least three parameters, cultured cells provided marked characteristics of EC phenotype, in HUVEC and in immortalized cell lines, regardless of their origin from the macro- or microcirculation. Toxicity experiments were assessed after 24 h exposure to cadmium, cyclosporin A and cisplatin by MTT assay. These experiments show nonsignificant difference in susceptibility to cyclosporin A and cadmium on HUVEC, HBMEC, and EA-hy-926. However, HBMEC, seems to be highly susceptible to cisplatin compared to HUVEC, the latter being more sensitive than EA-hy-926. For experiments conducted with cyclosporin and cadmium, cell lines could constitute an alternative material for routine cytotoxicity studies.

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Abbreviations

CsA:

cyclosporin A

EC:

endothelial cell

FCS:

fetal calf serum

HBMEC:

human bone marrow endothelial cell

HUVEC:

human umbilical cord vein endothelial cell

ICAM:

intracellular adhesion molecule

IMDM:

Iscove’s Modified Dulbecco’s Medium

MTT:

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PECAM:

platelet endothelial cell adhesion molecule

tPA:

tissue plasminogen activator

vWF:

von Willebrand factor

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Authors and Affiliations

  1. EA 3672, Université Victor Segalen Bordeaux 2, Bordeaux, F-33076, France

    B. L’Azou & J. Cambar

  2. INSERM, U577 Bordeaux, F-33076, France

    P. Fernandez, R. Bareille, M. Beneteau, C. Bourget & L. Bordenave

  3. Laboratoire Biologie Cellulaire, UFR Pharmacie Université, Victor Segalen Bordeaux 2, 146 rue Léo-Saignat, 33076, Bordeaux Cedex, France

    B. L’Azou

Authors
  1. B. L’Azou
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  2. P. Fernandez
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  3. R. Bareille
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  4. M. Beneteau
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  5. C. Bourget
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  6. J. Cambar
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  7. L. Bordenave
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Correspondence to B. L’Azou.

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L’Azou, B., Fernandez, P., Bareille, R. et al. In vitro endothelial cell susceptibility to xenobiotics: Comparison of three cell types. Cell Biol Toxicol 21, 127–137 (2005). https://doi.org/10.1007/s10565-005-0172-8

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  • DOI: https://doi.org/10.1007/s10565-005-0172-8

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