Abstract
Micro and nano fabrication techniques have facilitated the production of new devices for manipulation of single cells on a chip, such as the planar micro-pore electroporation technology. To characterize this technology we have studied the seal that forms at the interface between an individual cell and the micro-pore, in which the cell normally resides, as a function of an electrical field applied across the cell and temperature. Mathematical analysis of non-electroporative electrical fields in experiments with Madin-Darby canine kidney (MDCK) cells suggests that nanoscale channels form between the exterior of the cell and the pore wall. The results indicate that the electrical currents through these channels need to be considered when using planar micro-pores in general and performing micro-pore electroporation in particular. Our results show that the size of these channels is strongly temperature dependent and the cell to pore wall distance can increase by as much as 60% when the temperature of the system is lowered from 35 to 0∘C. Temperature appears to be an important factor in the use of devices for cells on a chip and our results suggest that physiological temperatures should yield better seal formation, thus improved feedback sensitivity, than the traditional use of room temperature in planar micro-pore electroporation devices.
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References
B. Alberts Molecular biology of the cell (Garland Science, New York, 2002), xxxiv.
E. Arzt and S. Gorband R. Spolenak, PNAS 100, 10603 (2003).
D. Braun and P. FromherzPhysical Review Letters 81, 5241 (1998).
D.C. Chang Guide to electroporation and electrofusion (Academic Press, San Diego, 1992), x.
D.P. Corey and C.F. Stevensin Single-channel recording, Edited by editoředitors (Plenum Press, New York, 1983), 53.
R. Dimova and B. Poulignyand C. Dietrich, Biophysical Journal 79, 340 (2000).
D.E. Discher and D.H. Boaland S.K. Boey, Biophysical Journal 75, 1584 (1998).
J.L. Drury and M. DemboBiophysical Journal 76, 110 (1999).
K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
M. Golzio and J. Teissieand M.P. Rols, Bioelectrochemistry 53, 25 (2001).
S. Grimnes and O.G. Martinsen Bioimpedance and bioelectricity basics (Academic, San Diego, Calif.; London, 2000), xiii.
K. Haas, W.C. Sin, A. Javaherian and Z. Liand H.T. Cline, Neuron 29, 583 (2001).
R.M. Hochmuth Journal of Biomechanics 33, 15 (2000).
Y. Huang and B. RubinskyBiomedical Microdevices 2, 145 (1999).
Y. Huang and B. RubinskySensors and Actuators a-Physical 89, 242 (2001).
Y. Huang and B. RubinskySensors and Actuators a-Physical 104, 205 (2003).
Y. Huang, N.S. Sekhon, J. Borninski and N. Chenand B. Rubinsky, Sensors and Actuators a-Physical 105, 31 (2003).
R.C. Lee and M.S. KolodneyPlastic and Reconstructive Surgery 80, 663 (1987).
R.C. Lee and D.J. Zhangand J. Hannig, Annual Review of Biomedical Engineering 2, 477 (2000).
J.A. Lundqvist, F. Sahlin, M.A.I. Aberg, A. Stromberg and P.S. Erikssonand O. Orwar, Proceedings of the National Academy of Sciences of the United States of America 95, 10356 (1998).
E. Neumann and A.E. Sowersand C.A. Jordan, Electroporation and electrofusion in cell biology (Plenum Press, New York, 1989), xviii.
K. Nolkrantz, C. Farre, A. Brederlau, R.I.D. Karlsson and C. BrennanP.S. Eriksson, S.G. Weber, M. Sandberg, and O. Orwar, Analytical Chemistry 73, 4469 (2001).
B.N.J. Persson, O. Albohr, U. Tartaglino and A.I. Volokitinand E. Tosatti, Journal of Physics-Condensed Matter 17, R1 (2005).
J.L. Rae and R.A. LevisPflugers Archiv-European Journal of Physiology 443, 664 (2002).
M.P. Rols et al., Nucleic Acids Research 22, 540 (1994).
A. Rosenberg Biology of the sialic acids (Plenum Press, New York, 1995), xv.
S. Sanchez and C. Guiand M. Elwenspoek, Journal of Micromechanics and Microengineering 7, 111 (1997).
A. Stett, V. Bucher, C. Burkhardt and U. Weberand W. Nisch, Medical & Biological Engineering & Computing 41, 233 (2003).
I.P. Sugár, J. Lindsay, and R.E. Schmukler, J. Phys. Chem. 107, 3862 (2003).
T.Y. Tsong Biophysical Journal 60, 297 (1991).
A.J. Wang and K.S. HuChin. Phys. Lett. 19, 1727 (2002).
J.C. Weaver Dielectrics and Electrical Insulation, IEEE Transactions on [see also Electrical Insulation, IEEE Transactions on] 10, 754 (2003).
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Díaz-Rivera, R.E., Rubinsky, B. Electrical and thermal characterization of nanochannels between a cell and a silicon based micro-pore. Biomed Microdevices 8, 25–34 (2006). https://doi.org/10.1007/s10544-006-6379-5
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DOI: https://doi.org/10.1007/s10544-006-6379-5