biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 60:55-67, 2016 | DOI: 10.1007/s10535-015-0569-z

Repetitive sequences in Eragrostis curvula cDNA EST libraries obtained from genotypes with different ploidy

J. Romero1, J. P. Selva1,2, S. Pessino3, V. Echenique1,4, I. Garbus1,5,*
1 Centro de Recursos Naturales Renovables de la Zona Semiárida, CONICET, Bahía Blanca, Argentina
2 Departamento de Biología Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina
3 Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina
4 Departamento de Agronomía, Universidad Nacional del Sur, Bahía Blanca, Argentina
5 Departamento de Ciencias de la Salud, Universidad Nacional del Sur, Bahía Blanca, Argentina

Eragrostis curvula (Schrad) Nees (weeping lovegrass) represents important cultivated forage in semiarid regions, and the most useful cultivars are tetraploid and reproduce by pseudogamous diplosporous apomixis. We previously produced a series of genetically related E. curvula lines that provide a suitable system for the identification of gene(s) involved in diplosporous apomixis and ploidy, including a natural apomictic tetraploid (T), a diploid sexual line (D), and a tetraploid sexual plant (C). A collection of expressed sequence tags (ESTs) was generated from cDNA libraries obtained from panicles of the D, T, and C, and leaves of the T. The present study aimed to analyze the repetitive content of these four cDNA libraries and further identify and characterize transposable element (TE)-related ESTs. Repetitive sequences were identified through the interface RepeatMasker (RM) using the database Repbase Update and further classification of TEs was performed manually from the RM output. The different contribution of ESTs with identity to TEs among libraries was further evaluated, and such differences were validated through RT-qPCR. We found that the percentage of repetitive content in the leaf cDNA library was almost double than in inflorescence libraries, with retrotransposons contributing mostly in all libraries. The expression of TE-related ESTs was compared in cDNA samples extracted from D, T, and C leaves or inflorescences revealing that seven mRNAs containing MuDR-like DNA transposons, Gypsy-like, and Copia-like retrotransposons were differentially represented according to tissue, reproductive mode, or ploidy. The euploid series of Eragrostis curvula is a useful model to the study of epigenomic changes produced after changes in ploidy. The present work constitutes the first detailed report on repetitive sequences of Eragrostis curvula at the transcriptome level.

Keywords: diplosporous apomixis; transposable elements; weeping lovegrass
Subjects: repetitive sequences; expressed sequence tags; ploidy; apomixis; transposable elements; gene expression; weeping lovegrass

Received: July 24, 2014; Revised: July 15, 2015; Accepted: September 2, 2015; Published: January 1, 2016  Show citation

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Romero, J., Selva, J.P., Pessino, S., Echenique, V., & Garbus, I. (2016). Repetitive sequences in Eragrostis curvula cDNA EST libraries obtained from genotypes with different ploidy. Biologia plantarum, 60(1), 55-67. doi: 10.1007/s10535-015-0569-z
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