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Recent research progress with phospholipase C from Bacillus cereus

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Abstract

Phospholipase C (PLC) catalyzes the hydrolysis of phospholipids to produce phosphate monoesters and diacylglycerol. It has many applications in the enzymatic degumming of plant oils. PLC Bc , a bacterial PLC from Bacillus cereus, is an optimal choice for this activity in terms of its wide substrate spectrum, high activity, and approved safety. Unfortunately, its large-scale production and reliable high-throughput screening of PLC Bc remain challenging. Herein, we summarize the research progress regarding PLC Bc with emphasis on the screening methods, expression systems, catalytic mechanisms and inhibitor of PLC Bc . This review hopefully will inspire new achievements in related areas, to promote the sustainable development of PLC Bc and its application.

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Acknowledgments

This work was supported by a Grant from Wilmar (Shanghai) Biotechnology Research and Development Center Co., Ltd.

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Authors and Affiliations

  1. Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People’s Republic of China

    Yan Lyu, Lidan Ye, Xiaohong Yang, Weiwei Chen & Hongwei Yu

  2. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People’s Republic of China

    Lidan Ye

  3. Wilmar (Shanghai) Biotechnology Research & Development Center Co. Ltd., No. 118 Gaodong Road, Pudong New District, Shanghai, 200137, People’s Republic of China

    Jun Xu

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  1. Yan Lyu
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  2. Lidan Ye
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  3. Jun Xu
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  5. Weiwei Chen
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  6. Hongwei Yu
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Correspondence to Hongwei Yu.

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Lyu, Y., Ye, L., Xu, J. et al. Recent research progress with phospholipase C from Bacillus cereus . Biotechnol Lett 38, 23–31 (2016). https://doi.org/10.1007/s10529-015-1962-6

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