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Nitric oxide is involved in the regulation of trehalose accumulation under heat stress in Pleurotus eryngii var. tuoliensis

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Abstract

Little is known about the mechanism of how trehalose responds to various abiotic stresses although trehalose is considered as an important protectant in fungi. We investigated the role of nitric oxide (NO) in regulating trehalose accumulation during heat stress in Pleurotus eryngii var. tuoliensis. The addition of 100 or 200 g trehalose/l significantly inhibited the production of thiobarbituric acid-reactive substance under heat stress in mycelial cells. High temperature induced endogenous trehalose accumulation and sodium nitroprusside, a NO donor, further enhanced trehalose accumulation. Finally, heat-induced trehalose accumulation could be arrested by the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1-1-oxyl-3-oxide, at 250 μM by inhibiting the transcription of trehalose phosphate synthase gene. Thus NO plays an important role in the regulation of trehalose accumulation during abiotic stresses in P. eryngii var. tuoliensis.

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Acknowledgments

This work was supported by the Special Fund for Agro-Scientific Research in the Public Interest (3–27) and China Agriculture Research System (CARS-24), Ministry of Agriculture of the People’s Republic of China.

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Authors and Affiliations

  1. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing, 100081, China

    Wei-Wei Kong, Chen-Yang Huang, Qiang Chen, Ya-Jie Zou, Meng-Ran Zhao & Jin-Xia Zhang

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  1. Wei-Wei Kong
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  2. Chen-Yang Huang
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  3. Qiang Chen
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  4. Ya-Jie Zou
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  5. Meng-Ran Zhao
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  6. Jin-Xia Zhang
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Corresponding author

Correspondence to Jin-Xia Zhang.

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Wei-Wei Kong and Chen-Yang Huang are contributed equally to this study.

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Kong, WW., Huang, CY., Chen, Q. et al. Nitric oxide is involved in the regulation of trehalose accumulation under heat stress in Pleurotus eryngii var. tuoliensis . Biotechnol Lett 34, 1915–1919 (2012). https://doi.org/10.1007/s10529-012-0988-2

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  • DOI: https://doi.org/10.1007/s10529-012-0988-2

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