Abstract
We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H+ transporter) and TF0F1-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATP-regenerating bioprocess for various ATP-hydrolyzing bioproductions.
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Acknowledgments
We thank Dr R. Biswas for critical reading of the manuscript. This study was supported by Grant-in-Aid for Young Scientists (B) (18769004, 22760608) and partially supported by “Establishment of Consolidated Research Institute for Advanced Science and Medical Care”, Encouraging Development Strategic Research Centers Program, the Special Coordination Funds for Promoting Science and Technology, Ministry of Education, Culture, Sports, Science and Technology, Japan. This study was also partially supported by Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan.
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Hara, K.Y., Suzuki, R., Suzuki, T. et al. ATP Photosynthetic vesicles for light-driven bioprocesses. Biotechnol Lett 33, 1133–1138 (2011). https://doi.org/10.1007/s10529-011-0544-5
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DOI: https://doi.org/10.1007/s10529-011-0544-5