Abstract
With the advent of antibody fragments and alternative binding scaffolds, that are devoid of Fc-regions, strategies to increase the half-life of small proteins are becoming increasingly important. Currently, the established method is chemical PEGylation, but more elaborate approaches are being described such as polysialylation, amino acid polymers and albumin-binding derivatives. This article reviews the main strategies for pharmacokinetic enhancement, primarily chemical conjugates and recombinant fusions that increase apparent molecular weight or hydrodynamic radius or interact with serum albumin which itself has a long plasma half-life. We highlight the key chemical linkage methods that preserve antibody function and retain stability and look forward to the next generation of technologies which promise to make better quality pharmaceuticals with lower side effects. Although restricted to antibodies, all of the approaches covered can be applied to other biotherapeutics.
Similar content being viewed by others
References
Abuchowski A, McCoy JR, Palczuk NC, van Es T, Davis FF (1977) Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase. J Biol Chem 252:3582–3586
Abuchowski A, Kazo GM, Verhoest CR Jr, Van Es T, Kafkewitz D, Nucci ML, Viau AT, Davis FF (1984) Cancer therapy with chemically modified enzymes. I. Antitumor properties of polyethylene glycol-asparaginase conjugates. Cancer Biochem Biophys 7:175–186
Adams GP, Shaller CC, Chappell LL, Wu C, Horak EM, Simmons HH, Litwin S, Marks JD, Weiner LM, Brechbiel MW (2000) Delivery of the alpha-emitting radioisotope bismuth-213 to solid tumors via single-chain Fv and diabody molecules. Nucl Med Biol 27:339–346
Aggarwal S (2008) What’s fueling the biotech engine—2007. Nat Biotechnol 26:1227–1233
Akhtar M, Al Mana H (2004) Molecular basis of proteinuria. Adv Anat Pathol 11:304–309
Andersen JT, Sandlie I (2009) The versatile MHC class I-related FcRn protects IgG and albumin from degradation: implications for development of new diagnostics and therapeutics. Drug Metab Pharmacokinet 24:318–332
Anderson CL, Chaudhury C, Kim J, Bronson CL, Wani MA, Mohanty S (2006) Perspective—FcRn transports albumin: relevance to immunology and medicine. Trends Immunol 27:343–348
Armstrong JK, Hempel G, Koling S, Chan LS, Fisher T, Meiselman HJ, Garratty G (2007) Antibody against poly(ethylene glycol) adversely affects PEG-asparaginase therapy in acute lymphoblastic leukemia patients. Cancer 110:103–111
Athwal D, Brown D, Weir A, Neil C, Popplewell A, Chapman A, King D (2001) Antibody molecules having specificity for human tumor necrosis factor alpha, and use thereof
Badger CC, Anasetti C, Davis J, Bernstein ID (1987) Treatment of malignancy with unmodified antibody. Pathol Immunopathol Res 6:419–434
Beauchamp CO, Gonias SL, Menapace DP, Pizzo SV (1983) A new procedure for the synthesis of polyethylene glycol-protein adducts; effects on function, receptor recognition, and clearance of superoxide dismutase, lactoferrin, and alpha 2-macroglobulin. Anal Biochem 131:25–33
Beckman RA, Weiner LM, Davis HM (2007) Antibody constructs in cancer therapy: protein engineering strategies to improve exposure in solid tumors. Cancer 109:170–179
Bendele A, Seely J, Richey C, Sennello G, Shopp G (1998) Short communication: renal tubular vacuolation in animals treated with polyethylene-glycol-conjugated proteins. Toxicol Sci 42:152–157
Bently M, Harris J (1999) Poly(ethylene glycol) aldehyde hydrates and related polymers and applications in modifying. US Patent 5,990,237
Bonavida B (2007) Rituximab-induced inhibition of antiapoptotic cell survival pathways: implications in chemo/immunoresistance, rituximab unresponsiveness, prognostic and novel therapeutic interventions. Oncogene 26:3629–3636
Borsi L, Balza E, Bestagno M, Castellani P, Carnemolla B, Biro A, Leprini A, Sepulveda J, Burrone O, Neri D, Zardi L (2002) Selective targeting of tumoral vasculature: comparison of different formats of an antibody (L19) to the ED-B domain of fibronectin. Int J Cancer 102:75–85
Carter PJ (2006) Potent antibody therapeutics by design. Nat Rev Immunol 6:343–357
Clark R, Olson K, Fuh G, Marian M, Mortensen D, Teshima G, Chang S, Chu H, Mukku V, Canova-Davis E, Somers T, Cronin M, Winkler M, Wells JA (1996) Long-acting growth hormones produced by conjugation with polyethylene glycol. J Biol Chem 271:21969–21977
Cohen O, Kronman C, Chitlaru T, Ordentlich A, Velan B, Shafferman A (2001) Effect of chemical modification of recombinant human acetylcholinesterase by polyethylene glycol on its circulatory longevity. Biochem J 357:795–802
Constantinou A, Epenetos AA, Hreczuk-Hirst D, Jain S, Deonarain MP (2008) Modulation of antibody pharmacokinetics by chemical polysialylation. Bioconjug Chem 19:643–650
Constantinou A, Epenetos AA, Hreczuk-Hirst D, Jain S, Wright M, Chester KA, Deonarain MP (2009) Site-specific polysialylation of an antitumor single-chain Fv fragment. Bioconjug Chem 19:643–650
Davies AJ (2007) Radioimmunotherapy for B-cell lymphoma: Y90 ibritumomab tiuxetan and I(131) tositumomab. Oncogene 26:3614–3628
Dennis MS, Zhang M, Meng YG, Kadkhodayan M, Kirchhofer D, Combs D, Damico LA (2002) Albumin binding as a general strategy for improving the pharmacokinetics of proteins. J Biol Chem 277:35035–35043
Dennis MS, Jin H, Dugger D, Yang R, McFarland L, Ogasawara A, Williams S, Cole MJ, Ross S, Schwall R (2007) Imaging tumors with an albumin-binding Fab, a novel tumor-targeting agent. Cancer Res 67:254–261
Deonarain MP (2008) Recombinant antibodies for cancer therapy. Expert Opin Biol Ther 8:1123–1141
Deonarain MP, Epenetos AA, Constaninou A (2008) WO2008025990. Biological materials and uses therof
Dolence E, Hu C, Tsang R, Sanders CG, Osaki S (1997) Electrophilic polyethylene oxides for the modification of polysaccharides peptides (proteins) and surfaces. US Patent 5,650,234
Dougan M, Dranoff G (2009) The immune response to tumors. Curr Protoc Immunol Chapter 20:Unit 20.11
Dumelin CE, Trussel S, Buller F, Trachsel E, Bootz F, Zhang Y, Mannocci L, Beck SC, Drumea-Mirancea M, Seeliger MW, Baltes C, Muggler T, Kranz F, Rudin M, Melkko S, Scheuermann J, Neri D (2008) A portable albumin binder from a DNA-encoded chemical library. Angew Chem Int Ed Engl 47:3196–3201
Ewert S, Honegger A, Pluckthun A (2004) Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering. Methods 34:184–199
Fee CJ (2007) Size comparison between proteins PEGylated with branched and linear poly(ethylene glycol) molecules. Biotechnol Bioeng 98:725–731
Fee CJ, Van Alstine JM (2004) Prediction of the viscosity radius and the size exclusion chromatography behavior of PEGylated proteins. Bioconjug Chem 15:1304–1313
Fernandes AI, Gregoriadis G (1996) Synthesis, characterization and properties of sialylated catalase. Biochim Biophys Acta 1293:90–96
Filpula D, Zhao H (2008) Releasable PEGylation of proteins with customized linkers. Adv Drug Deliv Rev 60:29–49
Francis GE, Fisher D, Delgado C, Malik F, Gardiner A, Neale D (1998) PEGylation of cytokines and other therapeutic proteins and peptides: the importance of biological optimisation of coupling techniques. Int J Hematol 68:1–18
Fraser-Moodie I (2008) Business Insights: delivery mechanisms for large molecule drugs: successes and failures of leading technologies and key drivers for market success
Fukumura D, Jain RK (2008) Imaging angiogenesis and the microenvironment. APMIS 116:695–715
Gaertner HF, Offord RE (1996) Site-specific attachment of functionalized poly(ethylene glycol) to the amino terminus of proteins. Bioconjug Chem 7:38–44
Georgopoulou N, Breen KC (1999) Overexpression of the alpha2,6 (N) sialyltransferase enzyme in human and rat neural cell lines is associated with increased expression of the polysialic acid epitope. J Neurosci Res 58:641–651
Ghetie V, Popov S, Borvak J, Radu C, Matesoi D, Medesan C, Ober RJ, Ward ES (1997) Increasing the serum persistence of an IgG fragment by random mutagenesis. Nat Biotechnol 15:637–640
Goodson RJ, Katre NV (1990) Site-directed pegylation of recombinant interleukin-2 at its glycosylation site. Biotechnology (N Y) 8:343–346
Graff CP, Wittrup KD (2003) Theoretical analysis of antibody targeting of tumor spheroids: importance of dosage for penetration, and affinity for retention. Cancer Res 63:1288–1296
Greenwald RB (2001) PEG drugs: an overview. J Control Release 74:159–171
Gregoriadis G, McCormack B, Wang Z, Lifely R (1993) Polysialic acids: potential in drug delivery. FEBS Lett 315:271–276
Gregoriadis G, Jain S, Papaioannou I, Laing P (2005) Improving the therapeutic efficacy of peptides and proteins: a role for polysialic acids. Int J Pharm 300:125–130
Gursahani H, Riggs-Sauthier J, Pfeiffer J, Lechuga-Ballesteros D, Fishburn CS (2009) Absorption of polyethylene glycol (PEG) polymers: the effect of PEG size on permeability. J Pharm Sci 98:2847–2856
Harris JM, Chess RB (2003) Effect of pegylation on pharmaceuticals. Nat Rev Drug Discov 2:214–221
Harris J, Herati R (1993) Preparation and use of poyethylene glycol propionaldehyde. US Patent 5,252,714
Harris J, Veronese F, Caliceti P, Schiavon O (1999) Multiarmed, monofunctional, polymer for coupling to molecules and surfaces. US Patent 5,932,462
Holliger P, Hudson PJ (2005) Engineered antibody fragments and the rise of single domains. Nat Biotechnol 23:1126–1136
Hudis CA (2007) Trastuzumab—mechanism of action and use in clinical practice. N Engl J Med 357:39–51
Huhalov A, Chester KA (2004) Engineered single chain antibody fragments for radioimmunotherapy. Q J Nucl Med Mol Imaging 48:279–288
Jain RK (1999) Transport of molecules, particles, and cells in solid tumors. Annu Rev Biomed Eng 1:241–263
Johansson MU, Frick IM, Nilsson H, Kraulis PJ, Hober S, Jonasson P, Linhult M, Nygren PA, Uhlen M, Bjorck L, Drakenberg T, Forsen S, Wikstrom M (2002) Structure, specificity, and mode of interaction for bacterial albumin-binding modules. J Biol Chem 277:8114–8120
Junutula JR, Raab H, Clark S, Bhakta S, Leipold DD, Weir S, Chen Y, Simpson M, Tsai SP, Dennis MS, Lu Y, Meng YG, Ng C, Yang J, Lee CC, Duenas E, Gorrell J, Katta V, Kim A, McDorman K, Flagella K, Venook R, Ross S, Spencer SD, Lee Wong W, Lowman HB, Vandlen R, Sliwkowski MX, Scheller RH, Polakis P, Mallet W (2008) Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index. Nat Biotechnol 26:925–932
Kaushik VV, Moots RJ (2005) CDP-870 (certolizumab) in rheumatoid arthritis. Expert Opin Biol Ther 5:601–606
Kinstler OB, Brems DN, Lauren SL, Paige AG, Hamburger JB, Treuheit MJ (1996) Characterization and stability of N-terminally PEGylated rhG-CSF. Pharm Res 13:996–1002
Kogan TP (1992) The synthesis of substituted methoxy-poly(ethylene glycol) derivatives suitable for selective protein modification. Synth Commun 22:2417–2424
Lee LS, Conover C, Shi C, Whitlow M, Filpula D (1999) Prolonged circulating lives of single-chain Fv proteins conjugated with polyethylene glycol: a comparison of conjugation chemistries and compounds. Bioconjug Chem 10:973–981
Lee S, Greenwald RB, McGuire J, Yang K, Shi C (2001) Drug delivery systems employing 1,6-elimination: releasable poly(ethylene glycol) conjugates of proteins. Bioconjug Chem 12:163–169
Linhult M, Binz HK, Uhlen M, Hober S (2002) Mutational analysis of the interaction between albumin-binding domain from streptococcal protein G and human serum albumin. Protein Sci 11:206–213
Miron T, Wilchek M (1993) A simplified method for the preparation of succinimidyl carbonate polyethylene glycol for coupling to proteins. Bioconjug Chem 4:568–569
Monfardini C, Schiavon O, Caliceti P, Morpurgo M, Harris JM, Veronese FM (1995) A branched monomethoxypoly(ethylene glycol) for protein modification. Bioconjug Chem 6:62–69
Morpurgo M, Veronese FM, Kachensky D, Harris JM (1996) Preparation of characterization of poly(ethylene glycol) vinyl sulfone. Bioconjug Chem 7:363–368
Muller D, Karle A, Meissburger B, Hofig I, Stork R, Kontermann RE (2007) Improved pharmacokinetics of recombinant bispecific antibody molecules by fusion to human serum albumin. J Biol Chem 282:12650–12660
Natarajan A, Xiong CY, Albrecht H, DeNardo GL, DeNardo SJ (2005) Characterization of site-specific ScFv PEGylation for tumor-targeting pharmaceuticals. Bioconjug Chem 16:113–121
Nguyen A, Reyes AE II, Zhang M, McDonald P, Wong WL, Damico LA, Dennis MS (2006) The pharmacokinetics of an albumin-binding Fab (AB.Fab) can be modulated as a function of affinity for albumin. Protein Eng Des Sel 19:291–297
Ober RJ, Radu CG, Ghetie V, Ward ES (2001) Differences in promiscuity for antibody–FcRn interactions across species: implications for therapeutic antibodies. Int Immunol 13:1551–1559
Panares RL, Garcia AA (2007) Bevacizumab in the management of solid tumors. Expert Rev Anticancer Ther 7:433–445
Pluckthun A, Pack P (1997) New protein engineering approaches to multivalent and bispecific antibody fragments. Immunotechnology 3:83–105
Presta LG (2008) Molecular engineering and design of therapeutic antibodies. Curr Opin Immunol 20:460–470
Roberts MJ, Bentley MD, Harris JM (2002) Chemistry for peptide and protein PEGylation. Adv Drug Deliv Rev 54:459–476
Roopenian DC, Akilesh S (2007) FcRn: the neonatal Fc receptor comes of age. Nat Rev Immunol 7:715–725
Sasahara S (1995) Temperature dependence of volume changes on glycine-PEG and L-alanine-PEG in aqueous solution. Colloid Polym Sci 273:782–786
Schlapschy M, Theobald I, Mack H, Schottelius M, Wester HJ, Skerra A (2007) Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life. Protein Eng Des Sel 20:273–284
Silbernagl S (1988) The renal handling of amino acids and oligopeptides. Physiol Rev 68:911–1007
Smith BJ, Popplewell A, Athwal D, Chapman AP, Heywood S, West SM, Carrington B, Nesbitt A, Lawson AD, Antoniw P, Eddelston A, Suitters A (2001) Prolonged in vivo residence times of antibody fragments associated with albumin. Bioconjug Chem 12:750–756
Stork R, Muller D, Kontermann RE (2007) A novel tri-functional antibody fusion protein with improved pharmacokinetic properties generated by fusing a bispecific single-chain diabody with an albumin-binding domain from streptococcal protein G. Protein Eng Des Sel 20:569–576
Strome SE, Sausville EA, Mann D (2007) A mechanistic perspective of monoclonal antibodies in cancer therapy beyond target-related effects. Oncologist 12:1084–1095
Thurber GM, Schmidt MM, Wittrup KD (2008) Factors determining antibody distribution in tumors. Trends Pharmacol Sci 29:57–61
Tijink BM, Laeremans T, Budde M, Stigter-van Walsum M, Dreier T, de Haard HJ, Leemans CR, van Dongen GA (2008) Improved tumor targeting of anti-epidermal growth factor receptor nanobodies through albumin binding: taking advantage of modular nanobody technology. Mol Cancer Ther 7:2288–2297
Tolmachev V, Orlova A, Pehrson R, Galli J, Baastrup B, Andersson K, Sandstrom M, Rosik D, Carlsson J, Lundqvist H, Wennborg A, Nilsson FY (2007) Radionuclide therapy of HER2-positive microxenografts using a 177Lu-labeled HER2-specific affibody molecule. Cancer Res 67:2773–2782
Tracey D, Klareskog L, Sasso EH, Salfeld JG, Tak PP (2008) Tumor necrosis factor antagonist mechanisms of action: a comprehensive review. Pharmacol Ther 117:244–279
Trussel S, Dumelin C, Frey K, Villa A, Buller F, Neri D (2009) A new strategy for the extension of the serum half-life of antibody fragments. Bioconjug Chem (Epub)
Veronese FM, Largajolli R, Boccu E, Benassi CA, Schiavon O (1985) Surface modification of proteins. Activation of monomethoxy-polyethylene glycols by phenylchloroformates and modification of ribonuclease and superoxide dismutase. Appl Biochem Biotechnol 11:141–152
Veronese FM, Caliceti P, Schiavon O (1997) Branched and linear poly(ethylene glycol): influence of the polymer structure on enzymological, pharmacokinetic and immunological properties of protein conjugates. J Bioact Compat Polym 12:196–207
Ward ES, Ober RJ (2009) Chapter 4: multitasking by exploitation of intracellular transport functions the many faces of FcRn. Adv Immunol 103:77–115
Woghiren C, Sharma B, Stein S (1993) Protected thiol-polyethylene glycol: a new activated polymer for reversible protein modification. Bioconjug Chem 4:314–318
Wright MJ, Deonarain MP (2007) Phage display of chelating recombinant antibody libraries. Mol Immunol 44:2860–2869
Yamaoka T, Tabata Y, Ikada Y (1994) Distribution and tissue uptake of poly(ethylene glycol) with different molecular weights after intravenous administration to mice. J Pharm Sci 83:601–606
Yamaoka T, Tabata Y, Ikada Y (1995) Fate of water-soluble polymers administered via different routes. J Pharm Sci 84:349–354
Yang K, Basu A, Wang M, Chintala R, Hsieh MC, Liu S, Hua J, Zhang Z, Zhou J, Li M, Phyu H, Petti G, Mendez M, Janjua H, Peng P, Longley C, Borowski V, Mehlig M, Filpula D (2003) Tailoring structure–function and pharmacokinetic properties of single-chain Fv proteins by site-specific PEGylation. Protein Eng 16:761–770
Yazaki PJ, Kassa T, Cheung CW, Crow DM, Sherman MA, Bading JR, Anderson AL, Colcher D, Raubitschek A (2008) Biodistribution and tumor imaging of an anti-CEA single-chain antibody–albumin fusion protein. Nucl Med Biol 35:151–158
Yokota T, Milenic DE, Whitlow M, Wood JF, Hubert SL, Schlom J (1993) Microautoradiographic analysis of the normal organ distribution of radioiodinated single-chain Fv and other immunoglobulin forms. Cancer Res 53:3776–3783
Youn YS, Na DH, Yoo SD, Song SC, Lee KC (2005) Carbohydrate-specifically polyethylene glycol-modified ricin A-chain with improved therapeutic potential. Int J Biochem Cell Biol 37:1525–1533
Zalipsky S, Barany G (1990) Facile synthesis of α-hydroxy-ω-carboxymethylpolyethylene oxide. J Bioact Compat Polym 5:227–231
Zalipsky S, Lee C (1992) Use of functionalized poly(ethylene glycol)s for modification of polypeptides. In: Harris JM, Zalipsky S (eds) Polyethylene glycol chemistry, biotechnical and biomedical applications. Plenum, New York, pp 347–370
Zalipsky S, Menon-Rudolph S (1997) Hydrazide derivatives of poly(ethylene glycol) and their bioconjugates. In: Harris S, Zalipsky S (eds) Poly(ethylene glycol) chemistry and biological applications. ACS Books, Washington, DC, pp 318–340
Zalipsky S, Seltzer R, Menon-Rudolph S (1992) Evaluation of a new reagent for covalent attachment of polyethylene glycol to proteins. Biotechnol Appl Biochem 15:100–114
Zalipsky S, Mullah N, Engbers C, Hutchins MU, Kiwan R (2007) Thiolytically cleavable dithiobenzyl urethane-linked polymer–protein conjugates as macromolecular prodrugs: reversible PEGylation of proteins. Bioconjug Chem 18:1869–1878
Acknowledgements
The authors acknowledge funding from Imperial College, Cancer Research UK (Ref: C18960) and European Union FP6 (Immuno-PDT Ref: LSHC-CT-2006-037489) which fund MPD, AC and CC respectively. We thank David Leak for critically reading the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Constantinou, A., Chen, C. & Deonarain, M.P. Modulating the pharmacokinetics of therapeutic antibodies. Biotechnol Lett 32, 609–622 (2010). https://doi.org/10.1007/s10529-010-0214-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-010-0214-z