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Dissecting cellular components of the secretory pathway in filamentous fungi: insights into their application for protein production

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Abstract

Studies on protein production using filamentous fungi have mostly focused on improvement of the protein yields by genetic modifications such as overexpression. Recent genome sequencing in several filamentous fungal species now enables more systematic approaches based on reverse genetics and molecular biology of the secretion pathway. In this review, we summarize recent molecular-based advances in our understanding of vesicular trafficking in filamentous fungi, and discuss insights into their high secretion ability and application for protein production.

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Acknowledgment

This work was supported by Grant-in-Aid for Scientific Research (S) and Grant-in-Aid for Scientific Research on Priority Areas “Applied Genomics” from the Ministry of Education, Culture, Sports, Science and Technology of Japan. Authors would like to thank M. Kuratsu and A. Taura for critical reading of the manuscript.

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  1. Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Jun-ya Shoji, Manabu Arioka & Katsuhiko Kitamoto

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  1. Jun-ya Shoji
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  2. Manabu Arioka
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  3. Katsuhiko Kitamoto
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Correspondence to Katsuhiko Kitamoto.

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Shoji, Jy., Arioka, M. & Kitamoto, K. Dissecting cellular components of the secretory pathway in filamentous fungi: insights into their application for protein production. Biotechnol Lett 30, 7–14 (2008). https://doi.org/10.1007/s10529-007-9516-1

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  • DOI: https://doi.org/10.1007/s10529-007-9516-1

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