Abstract
Efforts to increase H2 yields from fermentative H2 production include heat treatment of the inoculum, dissolved gas removal, and varying the organic loading rate. Although heat treatment kills methanogens and selects for spore-forming bacteria, the available evidence indicates H2 yields are not maximized compared to bromoethanesulfonate, iodopropane, or perchloric acid pre-treatments and spore-forming acetogens are not killed. Operational controls (low pH, short solids retention time) can replace heat treatment. Gas sparging increases H2 yields compared to un-sparged reactors, but no relationship exists between the sparging rate and H2 yield. Lower sparging rates may improve the H2 yield with less energy input and product dilution. The reasons why sparging improves H2 yields are unknown, but recent measurements of dissolved H2 concentrations during sparging suggest the assumption of decreased inhibition of the H2-producing enzymes is unlikely. Significant disagreement exists over the effect of organic loading rate (OLR); some studies show relatively higher OLRs improve H2 yield while others show the opposite. Discovering the reasons for higher H2 yields during dissolved gas removal and changes in OLR will help improve H2 yields.
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The authors would like to thank the William H. Doherty Ontario Graduate Scholarship in Science & Technology and the Natural Sciences and Engineering Research Council (NSERC) of Canada for funding.
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Kraemer, J.T., Bagley, D.M. Improving the yield from fermentative hydrogen production. Biotechnol Lett 29, 685–695 (2007). https://doi.org/10.1007/s10529-006-9299-9
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DOI: https://doi.org/10.1007/s10529-006-9299-9