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Identification of a Toll-Like Receptor 1 in Guinea Fowl (Agelastes niger)

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Abstract

Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns, thus playing important roles in host defense. This study determined the first sequence of a TLR1 type 1 in the guinea fowl (GFTLR1). The open reading frame of GFTLR1 type 1 contains 2,115 nucleotides and encodes 705 amino acids. Amino acid analysis indicated that GFTLR1 type 1 shares 92.3 % homology with the green jungle fowl, 92.1 % with the chicken, 90.4 % with the turkey, and 84.4 % with Cooper’s hawk. Genetic patterns were identified within the TLR1 type 1 of the chicken and the guinea fowl. GFTLR1 type 1 was found to have 92 polymorphic amino acid sites, of which 16 were in the leucine-rich repeat (LRR) domain, 3 in a C-terminal LRR domain, and 6 in a Toll/interleukin-1 receptor domain. The data showed that avian TLR1 type 1 genes are under purifying selection and highly conserved, because dN/dS was less than 1.

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Authors and Affiliations

  1. Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China

    Yanhua Wu

  2. College of Animal Science and Technology, Beijing University of Agriculture, Beijing, 102206, China

    Wenke Ruan, Defeng Cui & Huanrong Li

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  1. Yanhua Wu
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  2. Wenke Ruan
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  3. Defeng Cui
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  4. Huanrong Li
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Correspondence to Yanhua Wu or Wenke Ruan.

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Wu, Y., Ruan, W., Cui, D. et al. Identification of a Toll-Like Receptor 1 in Guinea Fowl (Agelastes niger). Biochem Genet 50, 702–716 (2012). https://doi.org/10.1007/s10528-012-9513-5

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  • DOI: https://doi.org/10.1007/s10528-012-9513-5

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