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Streptomyces polaris sp. nov. and Streptomyces septentrionalis sp. nov., isolated from frozen soil

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Abstract

Two novel actinomycetes, designated strains ZLN81T and ZLN712T, were isolated from a frozen soil sample which was collected from the Arctic region. Chemotaxonomic and morphological characteristics were found to be typical of members of the genus Streptomyces. Based on 16S rRNA gene sequence analyses, the two strains show high similarity with Streptomyces polygonati NEAU-G9T (99.45%, 99.17%) and Streptomyces yanglinensis 1307T (98.17%, 98.10%). DNA–DNA relatedness between each of the strains and their close phylogenetic neighbours showed that they belonged to distinct species. Multilocus sequence analysis (MLSA) using four housekeeping genes (atpD, gyrB, recA and rpoB) for comparing Streptomyces type strains showed that the MLSA distance of strains ZLN81T and ZLN712T to the closely related species was greater than the 0.007 threshold. The cell wall amino acids of the two strains were identified as alanine, glycine, asparagine, ll-diaminopimelic acid and meso-diaminopimelic acid. The whole cell sugars were identified as galactose and glucose for strain ZLN81T and galactose, glucose and xylose for strain ZLN712T. The predominant menaquinones were identified as MK-10(H8), MK-9(H4) and MK-9(H6) for strain ZLN81T and MK-9(H0), MK-10(H8) and MK-9(H6) for strain ZLN712T. The polar lipid profile of strain ZLN81T was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and two unidentified phospholipids, while that of strain ZLN712T consisted of diphosphatidylglycerol, phosphatidylethanolamine, a ninhydrin-positive glycophospholipid, phosphatidylinositol mannosides and two unidentified phospholipids. The major fatty acids were identified as iso-C16:0, anteiso-C17:0, C16:0 and anteiso-C15:0 for strain ZLN81T and iso-C16:0, C16:0, anteiso-C15:0 and iso-C14:0 for strain ZLN712T. The DNA G+C contents were found to be 73.96 and 72.77 mol% for strains ZLN81T and ZLN712T, respectively. On the basis of these phenotypic and genotypic data, strains ZLN81T (= CCTCC AA 2018010T = DSM 107255T) and ZLN712T (= CCTCC AA 2018011T = DSM 107266T) are concluded to represent two novel species of the genus Streptomyces, for which the names Streptomyces polaris sp. nov. and Streptomyces septentrionalis sp. nov. are proposed, respectively.

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Funding

This research was supported by the National Key Research and Development Program of China 2018YFC0311000 and the EU FP7 Project PharmaSea (312184).

Author contributions

MK performed the laboratory experiments, analyzed the data, and drafted the manuscript. PN contributed to the polyphasic taxonomy. LZ contributed to the isolation of Arctic actinomycete strains and primarily identified that the two strains may be novel species. FP contributed to the collection of frozen soil from the Arctic. KH and ZD conceived the experiments and revised the whole manuscript.

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Authors and Affiliations

  1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, People’s Republic of China

    Manita Kamjam, Potjanicha Nopnakorn, Li Zhang, Zixin Deng & Kui Hong

  2. China Center for Type Culture Collection, Wuhan, 430071, People’s Republic of China

    Fang Peng

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  1. Manita Kamjam
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  2. Potjanicha Nopnakorn
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  3. Li Zhang
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  4. Fang Peng
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Correspondence to Kui Hong.

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Kamjam, M., Nopnakorn, P., Zhang, L. et al. Streptomyces polaris sp. nov. and Streptomyces septentrionalis sp. nov., isolated from frozen soil. Antonie van Leeuwenhoek 112, 375–387 (2019). https://doi.org/10.1007/s10482-018-1166-x

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