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Streptomyces formicae sp. nov., a novel actinomycete isolated from the head of Camponotus japonicus Mayr

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Abstract

During a screening for novel and biotechnologically useful actinobacteria in insects, a novel actinomycete with antifungal activity, designated strain 1H-GS9T, was isolated from the head of a Camponotus japonicus Mayr ant, which were collected from Northeast Agricultural University (Harbin, Heilongjiang, China). Strain 1H-GS9T was characterised using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of members of the genus Streptomyces. 16S rRNA gene sequence similarity studies showed that strain 1H-GS9T belongs to the genus Streptomyces with high sequence similarities to Streptomyces scopuliridis DSM 41917T (98.8 %) and Streptomyces mauvecolor JCM 5002T (98.6 %). However, phylogenetic analysis based on the 16S rRNA gene sequence indicated that it forms a monophyletic clade with Streptomyces kurssanovii JCM 4388T (98.6 %), Streptomyces xantholiticus JCM 4282T (98.6 %) and Streptomyces peucetius JCM 9920T (98.5 %). Thus, a combination of DNA–DNA hybridization experiments and phenotypic tests were carried out between strain 1H-GS9T and the above-mentioned five strains, which further clarified their relatedness and demonstrated that strain 1H-GS9T could be distinguished from these strains. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces formicae sp. nov. is proposed. The type strain is 1H-GS9T (=CGMCC 4.7277T = DSM 100524T).

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Acknowledgments

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Natural Science Foundation of China (No. 31471832, 31171913, 31500010, 31572070 and 31372006), the National Key Technology R&D Program (No. 2012BAD19B06), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP), the Youth Science Foundation of Heilongjiang Province (No. QC2014C013), the “Young Talents” Project of Northeast Agricultural University (14QC02), the Sicence and Technology Research Project of Heilongjiang Provincial Educational Commission (No. 12541001), the China Postdoctoral Science Foundation (2014M561319) and the Heilongjiang Postdoctoral Fund (LBH-Z14027). We are grateful to Prof. Aharon Oren for helpful advice on the specific epithet.

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    1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

      Lu Bai, Chongxi Liu, Lifeng Guo, Chenyu Piao, Zhilei Li, Jiansong Li, Feiyu Jia, Xiangjing Wang & Wensheng Xiang

    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

      Wensheng Xiang

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    1. Lu Bai
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    Correspondence to Xiangjing Wang or Wensheng Xiang.

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    Lu Bai and Chongxi Liu have contributed equally to this work.

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    Bai, L., Liu, C., Guo, L. et al. Streptomyces formicae sp. nov., a novel actinomycete isolated from the head of Camponotus japonicus Mayr. Antonie van Leeuwenhoek 109, 253–261 (2016). https://doi.org/10.1007/s10482-015-0628-7

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