Abstract
A Gram-stain negative, non-motile, rod-shaped and aerobic bacterial strain, designated JG48T, was isolated from a gravel sample taken from a beach adjacent to Udo island, South Korea. Strain JG48T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The 16S rRNA gene sequence of strain JG48T exhibited sequence similarities of 96.67 % to Hyunsoonleella jejuensis CNU004T. The major fatty acids present in the strain JG48T were identified as iso-C15:0, iso-C15:1 G, iso-C17:0 3–OH and iso-C15:0 3–OH. The predominant isoprenoid quinone was identified as MK-6. The polar lipids profile of strain JG48T was found to consist of phosphatidylethanolamine, three unidentified amino lipids and four unidentified lipids. The DNA G+C content of strain JG48T was determined to be 34 mol%. Based on the morphological and physiological properties, and the results of phylogenetic analyses, the strain is considered to represent a novel species of the genus Hyunsoonleella, for which the name Hyunsoonleella udoensis sp. nov. is proposed. The type strain is JG48T (=KCTC 42341T=JCM 30600T).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bernardet JF, Nakagawa Y (2006) An introduction to the family Flavobacteriaceae. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The prokaryotes: a handbook on the biology of bacteria, vol 7, 3rd edn. Springer, New York, pp 455–480
Bernardet JF, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070
Fautz E, Reichenbach H (1980) A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Felsenstein J (1993) PHYLIP (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle
Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Patel PV, Alshamaony L (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
Moore DD, Dowhan D (1995) Preparation and analysis of DNA. In: Ausubel FW, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley, New York, pp 2–11
Park SC, Choe HN, Hwang YM, Baik KS, Kim SN, Lee YS, Jung JS, Seong CN (2013) Marinivirga aestuarii gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from marine environments, and emended descriptions of the genera Hyunsoonleella, Jejuia and Pontirhabdus and the species Hyunsoonleella jejuensis, Jejuia pallidilutea and Pontirhabdus pectinivorans. Int J Syst Evol Microbiol 63:1524–1531
Reichenbach H (1989) The order Cytophagales Leadbetter 1974, 99AL. In: Staley JT, Bryant MP, Pfennig N, Holt JC (eds) Bergey’s manual of systematic bacteriology, vol 3. Williams and Wilkins, Baltimore, pp 2011–2073
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York
Sasser M (2001) Identification of bacteria by gas chromatography of cellular fatty acids. Technical note 101. MIDI Inc., Newark
Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Tittsler RP, Sandholzer LA (1936) The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31:575–580
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
Yoon BJ, Lee DH, Kang BJ, Kahng HY, Oh YS, Sohn JH, Choi ES, Oh DC (2010) Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 60:382–386
Acknowledgments
This work was supported by a grant from the National Fisheries Research and Development Institute (R2015028), and a Grant from the KRIBB Research Initiative Program (KGM4111551).
Author information
Authors and Affiliations
Corresponding author
Additional information
Hyangmi Kim and Jae-Bong Lee have contributed equally to this study.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Kim, H., Lee, JB., Oh, HW. et al. Hyunsoonleella udoensis sp. nov., isolated from a gravel sample from a beach of Udo island, Korea. Antonie van Leeuwenhoek 108, 1009–1014 (2015). https://doi.org/10.1007/s10482-015-0553-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-015-0553-9