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Sphaerisporangium corydalis sp. nov., isolated from the root of Corydalis yanhusuo L.

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Abstract

Two Gram-stain positive, aerobic actinomycete strains, designated NEAU-YHS12 and NEAU-YHS15T, were isolated from the root of Corydalis yanhusuo L. collected from Wuchang, Heilongjiang Province, northeast China. Phylogenetic analysis of 16S rRNA gene sequences showed that the two strains are closely related to one another (99.8 % similarity), and had the closest relationship with Sphaerisporangium cinnabarinum JCM 3291T (98.7, 98.6 %), Sphaerisporangium flaviroseum YIM 48771T (98.6, 98.6 %), Sphaerisporangium melleum JCM 13064T (98.5, 98.4 %) and Sphaerisporangium dianthi NEAU-CY18T (98.4, 98.4 %). DNA–DNA hybridization value between strains NEAU-YHS12 and NEAU-YHS15T was 82 ± 1.4 %, and the values between the two strains and the closely related type strains were well below 70 %. The two strains also shared a number of phenotypic characteristics that were distinct from the closely related species. Both strains were observed to contain MK-9(H6), MK-9(H4) and MK-9(H2) as the detected menaquinones. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The phospholipid profiles were found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, phosphoglycolipid, and an unidentified phospholipid. The major fatty acids were identified as iso-C16:0, C17:1 ω7c, C18:0 and iso-C15:0. On the basis of the genetic and phenotypic properties, it is proposed that strains NEAU-YHS15T and NEAU-YHS12 be classified as representatives of a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium corydalis sp. nov is proposed. The type strain is NEAU-YHS15T (CGMCC 4.7148T = DSM46732T).

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Acknowledgments

This work was supported in part by Grants from the National Outstanding Youth Foundation (No. 31225024), the National Natural Science Foundation of China (No. 31471832, 31171913 and 31372006), the National Key Technology R&D Program (No. 2012BAD19B06), the Program for New Century Excellent Talents in University (NCET-11-0953), the Outstanding Youth Foundation of Heilongjiang Province (JC201201), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP), the China Postdoctoral Science Foundation (2014M561319) and the Heilongjiang Postdoctoral Fund (LBH-Z14027).

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang, Harbin, 150030, People’s Republic of China

    Xin Wang, Chongxi Liu, Yuejing Zhang, Zhaoxu Ma, Lianjie Li, Xiangjing Wang & Wensheng Xiang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Jiaxu Cheng & Wensheng Xiang

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  1. Xin Wang
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Wang, X., Liu, C., Cheng, J. et al. Sphaerisporangium corydalis sp. nov., isolated from the root of Corydalis yanhusuo L.. Antonie van Leeuwenhoek 108, 133–139 (2015). https://doi.org/10.1007/s10482-015-0471-x

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