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Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations

  • Clinical Investigation
  • Published:
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Abstract

Purpose

To report the use of hyperdried cross-linked (HDCL) amniotic membrane (AM) patching with tissue adhesive as an initial therapy for corneal perforations.

Methods

Cryopreserved AM was cross-linked with 0.1% glutaraldehyde and then dried using far-infrared rays and microwaves (hyperdry method). Three eyes of three patients with corneal perforations of up to 3 mm in diameter were included in this study. They were treated with a single-layer patch of HDCL-AM applied with a tissue adhesive (2-octyl-cyanoacrylate). We also evaluated the resistance of HDCL-AM to collagenases during in vitro digestion testing.

Results

In all three cases, the corneal perforations were repaired within 28 days (range, 17–28 days). No recurrence occurred during the follow-up period (3–6 months). In the collagenase digestion testing, the HDCL-AM did not dissolve until 48 h, whereas the cryopreserved AM completely dissolved within 60 min.

Conclusions

Three cases of corneal perforations were successfully managed using HDCL-AM patching with tissue adhesive. The HDCL-AM was resistant to collagenases during in vitro digestion testing. The HDCL-AM was a useful substrate for corneal perforations. This simple surgical technique may be one of the initial therapeutic options for corneal perforations.

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Authors and Affiliations

  1. Department of Ophthalmology, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, Japan

    Kiyotaka Kitagawa, Shuichiro Yanagisawa, Xue-Yun Zhang & Atsushi Hayashi

  2. Department of Regenerative Medicine, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, Japan

    Motonori Okabe & Toshio Nikaido

  3. Department of Ophthalmology, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, 2630 Sugitani, Toyama, 930-0194, Japan

    Atsushi Hayashi

Authors
  1. Kiyotaka Kitagawa
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  2. Motonori Okabe
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  3. Shuichiro Yanagisawa
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  4. Xue-Yun Zhang
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  5. Toshio Nikaido
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  6. Atsushi Hayashi
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Corresponding author

Correspondence to Atsushi Hayashi.

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Kitagawa, K., Okabe, M., Yanagisawa, S. et al. Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations. Jpn J Ophthalmol 55, 16–21 (2011). https://doi.org/10.1007/s10384-010-0903-0

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  • DOI: https://doi.org/10.1007/s10384-010-0903-0

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