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Magnetic field-induced oxidative stress and DNA damage in Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

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Abstract

Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) is cosmopolitan pest of stored products. The effect of strong magnetic fields (MFs) on DNA damage and oxidative stress on larvae stage of E. kuehniella was assessed. Antioxidant enzyme systems, which include superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and malondialdehyde (MDA), the end product of lipid peroxidation as a result of strong MF intoxication that might occur in the larvae tissue, were evaluated. A simple technique of single-cell gel electrophoresis (DNA comet assay) enabled a quick detection of MF treatment on larvae. The larvae were exposed in a 1.4-Tesla (T) MF from a DC power supply at 50 Hz for different time periods (3, 6, 12, 24, 48, and 72 h). MFs caused increasing DNA damage and demonstrated using the comet assay with its parameters including tail DNA%, tail length and tail moment. DNA damage at increasing exposure times were significantly larger than the control group (p < 0.05). These parameters were detected using BS 200 ProP with image analysis software. SOD, CAT, GPx, and GST activities decreased and MDA level increased in the MF-treated group in larvae tissue compared to control group for increasing exposure times at 1.4 T (p < 0.05). In our investigation, we showed that MFs caused oxidative stress and proved to be DNA damage as revealed by the comet assay. MFs may be used to determine potential toxic effects as a control agent against E. kuehniella larvae.

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Acknowledgments

The authors would like to thank Bozok University Science and Technology Application and Research Center for supplying the Hall Effects System and Mrs. Sevcan Oztemiz (Adana Plant Protection Research Institute) for supplying the Ephestia kuehniella used in the experiments.

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  1. Department of Biology, Faculty of Arts and Science, Bozok University, 66100, Yozgat, Turkey

    Dilek Pandir

  2. Department of Physics, Faculty of Arts and Science, Bozok University, Yozgat, Turkey

    Recep Sahingoz

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  1. Dilek Pandir
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  2. Recep Sahingoz
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Correspondence to Dilek Pandir.

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Communicated by C.G. Athanassiou.

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Pandir, D., Sahingoz, R. Magnetic field-induced oxidative stress and DNA damage in Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae. J Pest Sci 87, 79–87 (2014). https://doi.org/10.1007/s10340-013-0521-y

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