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Exosomal miR-1246 in serum as a potential biomarker for early diagnosis of gastric cancer

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International Journal of Clinical Oncology Aims and scope Submit manuscript

Abstract

Background

Gastric cancer (GC) patients are usually diagnosed in advanced stages which results in high mortality. This study aimed to identify novel circulating miRNAs as biomarkers for the early detection of GC.

Methods

Candidate miRNA was identified after integrated analysis of two Gene Expression Omnibus (GEO) datasets and clinical serum samples. Exosomes extracted were verified using transmission electron microscopy (TEM) and western blot. The expressions of miRNAs were tested through qRT-PCR. Receiver operating characteristic curve (ROC) analysis was used to explore the diagnostic utility of miRNAs. RNA pull-down assay was used to find RNA binding proteins (RBPs) which transport candidate miRNA into exosomes. Bioinformatics analysis of candidate miRNA was conducted using DAVID and Cytoscape.

Results

After integrated analysis of two GEO datasets, six circulating miRNAs were found to be consistently upregulated in GC patients. Then, qRT-PCR demonstrated that serum miR-1246 was the one with the largest fold change. Studies in vitro revealed that elevated serum miR-1246 was tumor-derived by being packaged into exosomes with the help of ELAVL1. Thereafter, we discovered that exosomal miR-1246 expressions in serum could differentiate GC patients with TNM stage I from healthy controls (HCs) and patients with benign diseases (BDs) with area under the curve (AUC) of 0.843 and 0.811, respectively. Bioinformatics analysis revealed miR-1246, as a tumor suppressor in GC, could regulate several signaling pathways.

Conclusion

Circulating exosomal miR-1246 was a potential biomarker for the early diagnosis of GC.

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Author information

Author notes

  1. Yuntao Shi and Zhonghong Wang contribute to the study equally.

Authors and Affiliations

  1. Medical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, No.121, Jiangjiayuan Road, Nanjing, 210011, China

    Yuntao Shi, Xiaojuan Zhu, Ling Chen, Yilan Ma, Jiayan Wang & Zheng Liu

  2. Department of Gastroenterology, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, No.1, Huanghe West Road, Huaian, 223300, China

    Yuntao Shi & Xiaozhong Yang

  3. Department of Gastroenterology, Huaian People’s Hospital of Hongze District, Huaian, 223100, China

    Zhonghong Wang

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  1. Yuntao Shi
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  2. Zhonghong Wang
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  3. Xiaojuan Zhu
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  4. Ling Chen
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Correspondence to Xiaozhong Yang or Zheng Liu.

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This study was approved by the Research and Ethical Committee of The Second Affiliated Hospital of Nanjing Medical University.

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Shi, Y., Wang, Z., Zhu, X. et al. Exosomal miR-1246 in serum as a potential biomarker for early diagnosis of gastric cancer. Int J Clin Oncol 25, 89–99 (2020). https://doi.org/10.1007/s10147-019-01532-9

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  • DOI: https://doi.org/10.1007/s10147-019-01532-9

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