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Photobiomodulation and diffusing optical fiber on spinal cord’s impact on nerve cells from normal spinal cord tissue in piglets

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Abstract

Experts have proven that photobiological regulation therapy for spinal cord injury promotes the spinal repair following injury. The traditional irradiation therapy mode is indirect (percutaneous irradiation), which could significantly lower the effective use of light energy. In earlier studies, we developed an implantable optical fiber that one can embed above the spinal cord lamina, and the light directly is cast onto the surface of the spinal cord in a way that can dramatically improve energy use. Nonetheless, it remains to be seen whether near-infrared light diffused by embedded optical fiber can have side effects on the surrounding nerve cells. Given this, we implanted optical fiber on the lamina of a normal spinal cord to observe the structural integrity of the tissue using morphological staining; we also used immunohistochemistry to detect inflammatory factors. Considering the existing studies, we meant to determine that the light energy diffused by embedded optical fiber has no side effect on the normal tissue. The results of this study will lay a foundation for the clinical application of the treatment of spinal cord injury by near-infrared light irradiation.

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Funding

A National Natural Scientific Foundation of China Grant (NO. 81572151) and the key research and development program in Social Development of Shaanxi Province (grant number 2020ZDLSF02-05) and the Key Science and Technology Program in Social Development of Shaanxi Province (grant number 2016SF-012, 2017SF-021) supported the current study.

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Author notes

  1. Xiaoshuang Zuo, Zhuowen Liang and Jiawei Zhang contributed equally to this work.

Authors and Affiliations

  1. Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, Shaanxi, China

    Xiaoshuang Zuo, Zhuowen Liang, Jiawei Zhang, Qiao Zheng, Yangguang Ma, Penghui Li, Tan Ding, Xueyu Hu & Zhe Wang

  2. Institute of Photonics and Photon-Technology, Northwest University, Xi’an, 710032, Shaanxi, China

    Shuang Wang

Authors
  1. Xiaoshuang Zuo
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  2. Zhuowen Liang
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  3. Jiawei Zhang
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  4. Shuang Wang
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  5. Qiao Zheng
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  6. Yangguang Ma
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  7. Penghui Li
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  8. Tan Ding
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  9. Xueyu Hu
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  10. Zhe Wang
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Corresponding authors

Correspondence to Tan Ding, Xueyu Hu or Zhe Wang.

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The authors declare that they have no conflict of interest.

Ethics approval

All animal care and experimental programs were conducted according to standard All applicable international and national ethical guidelines (National Institutes of Health Guide to the use of Laboratory Animals) and approved by The Animal Care Ethics Committee of the Fourth Military Medical University (KY20172036-I).

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Zuo, X., Liang, Z., Zhang, J. et al. Photobiomodulation and diffusing optical fiber on spinal cord’s impact on nerve cells from normal spinal cord tissue in piglets. Lasers Med Sci 37, 259–267 (2022). https://doi.org/10.1007/s10103-020-03231-8

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  • DOI: https://doi.org/10.1007/s10103-020-03231-8

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