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Effect of 830-nm diode laser irradiation on human sperm motility

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Abstract

Sperm motility is known as an effective parameter in male fertility, and it depends on energy consumption. Low-level laser irradiation could increase energy supply to the cell by producing adenosine triphosphate. The purpose of this study is to evaluate how the low-level laser irradiation affects the human sperm motility. Fresh human semen specimens of asthenospermic patients were divided into four equal portions and irradiated by 830-nm GaAlAs laser irradiation with varying doses as: 0 (control), 4, 6 and 10 J/cm2. At the times of 0, 30, 45 and 60 min following irradiation, sperm motilities are assessed by means of computer-aided sperm analysis in all samples. Two additional tests [HOS and sperm chromatin dispersion (SCD) tests] were also performed on the control and high irradiated groups as well. Sperm motility of the control groups significantly decreased after 30, 45 and 60 min of irradiation, while those of irradiated groups remained constant or slightly increased by passing of time. Significant increases have been observed in doses of 4 and 6 J/cm2 at the times of 60 and 45 min, respectively. SCD test also revealed a non-significant difference. Our results showed that irradiating human sperms with low-level 830-nm diode laser can improve their progressive motility depending on both laser density and post-exposure time.

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Acknowledgments

We would like to thank the all staffs and patients of Royan Institute Clinical Laboratory for providing us the semen samples and analysing facilities, especially laboratory technicians Abdol-Ali Ansary and Kaveh Afraz.

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Authors and Affiliations

  1. Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

    Reza Salman Yazdi, Firooz Jannat Alipoor, Faramarz Farrahi & Mohammad Ali Sadighi Gilani

  2. LASER Department, Golha Clinic, Tehran, Iran

    Simin Bakhshi

  3. Department of Epidemiology and Reproductive Health at Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

    Mohammad Reza Akhoond & Mehdi Lotfi Panah

  4. Shahid Beheshti University of Medical Science, Tehran, Iran

    Soheila Borhani

  5. Royan Institute, No.12 East-Hafez St., North Bani-Hashem St., Resalat Ave, Tehran, Iran

    Reza Salman Yazdi

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  1. Reza Salman Yazdi
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  2. Simin Bakhshi
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  3. Firooz Jannat Alipoor
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Correspondence to Reza Salman Yazdi.

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Salman Yazdi, R., Bakhshi, S., Jannat Alipoor, F. et al. Effect of 830-nm diode laser irradiation on human sperm motility. Lasers Med Sci 29, 97–104 (2014). https://doi.org/10.1007/s10103-013-1276-7

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  • DOI: https://doi.org/10.1007/s10103-013-1276-7

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