Abstract
Enzymatic saccharifications of the hydrolysate collected as the water-soluble (WS) portion after hot-compressed water (HCW) treatment of Japanese beech with xylanase and β-xylosidase were performed to investigate the hydrolytic properties of these enzymes. The WS portions with different molecular weight distributions and initial concentrations of xylooligosaccharides were prepared as substrates, one of which contained mainly smaller xylooligosaccharides [degree of polymerization (DP) = 2 or 3], while the other contained larger xylooligosaccharides (DP ≥ 4). The highest xylose recovery was obtained from both of the WS portions treated with β-xylosidase rather than with xylanase. This suggests that β-xylosidase could have a higher activity than xylanase toward larger xylooligosaccharides (DP ≥ 4) as well as smaller xylooligosaccharides (DP = 2 or 3) recovered from HCW treatment. As a result, the use of β-xylosidase was found to be effective for saccharifi cation of the WS portion, even with a short reaction time for the HCW treatment.
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Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour Technol 96:673–686
Mok WS, Antal MJJr (1992) Uncatalyzed solvolysis of whole biomass hemicellulose by hot compressed liquid water. Ind Eng Chem Res 31:1157–1161
Yang B, Wyman CE (2004) Effect of xylan and lignin removal by batch and fl owthrough pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnol Bioeng 86:88–95
Laser M, Schulman D, Allen SG, Lichwa J, Antal MJJr, Lynd LR (2002) A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol. Bioresour Technol 81:33–44
Selig MJ, Knoshaug EP, Adney WS, Himmel ME, Decker SR (2008) Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities. Bioresour Technol 99:4997–5005
Liu C, Wyman CE (2003) The effect of fl ow rate of compressed hot water on xylan, lignin, and total mass removal from corn stover. Ind Eng Chem Res 42:5409–5416
Collins T, Gerday C, Feller G (2005) Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 29:3–23
Polizeli MLTM, Rizzatti ACS, Monti R, Terenzi HF, Jorge JA, Amorim DS (2005) Xylanases from fungi: properties and industrial applications. Appl Microbiol Biotechnol 67:577–591
Kolenová K, Vršanská M, Biely P (2006) Mode of action of endo-β-1,4-xylanases of families 10 and 11 on acidic xylooligosaccharides. J Biotechnol 121:338–345
Wong KKY, Tan LUL, Saddler JN (1988) Multiplicity of β-1,4-xylanase in microorganisms: functions and applications. Microbiol Rev 52:305–317
Royer JC, Nakas JP (1991) Purification and characterization of two xylanases from Trichoderma longibrachiatum. Eur J Biochem 202:521–529
Takenishi S, Tsujisaka Y, Fukumoto J (1973) Purification and properties of the β-xylosidase produced by Aspergillus niger van Tieghem. J Biochem 73:335–343
Puls J, Tenkanen M, Korte HE, Poutanen K (1991) Products of hydrolysis of beechwood acetyl-4-O-methylglucuronoxylan by a xylanase and an acetyl xylan esterase. Enzym Microb Technol 13:483–486
Puls J, Schmidt O, Granzow C (1986) α-Glucuronidase in two microbial xylanolytic systems. Enzym Microb Technol 9: 83–88
Saka S, Ueno T (1999) Chemical conversion of various celluloses to glucose and its derivatives in supercritical water. Cellulose 6:177–191
Ehara K, Saka S (2002) A comparative study on chemical conversion of cellulose between the batch-type and flow-type systems in supercritical water. Cellulose 9:301–311
Poutanen K, Rättü M, Puls J, Viikari L (1987) Evaluation of different microbial xylanolytic systems. J Biotechnol 6:49–60
Poutanen K, Puls J (1988) Characteristics of Trichoderma reesei β-xylosidase and its use in the hydrolysis of solubilized xylans. Appl Microbiol Biotechnol 28:425–432
Kaya F, Heitmann JA, Joyce TW (2000) Influence of lignin and its degradation products on enzymatic hydrolysis of xylan. J Biotechnol 80:241–247
Sharma A, Milstein O, Vered Y, Gressel J (1985) Effects of aromatic compounds on hemicellulose-degrading enzymes in Aspergillus japonicus. Biotechnol Bioeng 27:1095–1101
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Nakata, T., Miyafuji, H. & Saka, S. Enzymatic saccharification of water-soluble portion after hot-compressed water treatment of Japanese beech with xylanase and β-xylosidase. J Wood Sci 55, 209–214 (2009). https://doi.org/10.1007/s10086-008-1018-9
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DOI: https://doi.org/10.1007/s10086-008-1018-9