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Enzymatic extracts from edible red algae, Porphyra tenera, and their antioxidant, anti-acetylcholinesterase, and anti-inflammatory activities

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Abstract

Enzymatic extracts from Porphyra tenera were prepared using 4 proteases (Protamex, Neutrase, Flavourzyme, and Alcalase) and 7 carbohydrases (AMG, Celluclast, Dextrozyme, Maltogenase, Termamyl, Promozyme, and Viscozyme), and biological activities of the enzymatic extracts from P. tenera were determined as antioxidant, anti-acetylcholinestrase (AChE), and anti-inflammation. The Alcalase and Maltogenase extracts showed higher 1,1-diphenyl-2-picrylhydrozyl (DPPH) scavenging activities compared to the other extracts. At the 2.5 mg/mL, 94.38% (Alacalase extracts), and 80.13% (Maltogenase extracts) scavenging capacities were observed. The Alcalase and Maltogenase extracts were also showed strong reducing power, ferrous ion chelating, and hydrogen peroxide (H2O2) scavenging capacities. In addition, 2 enzymatic extracts effectively protected hydroxyl radical-induced DNA damage. In the case of AChE inhibition, the Flavourzyme (99.32% inhibition) and Viscozyme extracts (82.68% inhibition) were observed. All enzymatic extracts showed no cytotoxicity in RAW264.7 macrophages, and all enzymatic extracts effectively inhibited lipopolysaccharides (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophages. These results suggest that the enzymatic extracts from P. tenera would be useful as an ingredient for functional foods.

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Authors and Affiliations

  1. School of Food Technology and Nutrition, Chonnam National University, Yeosu, Jeonnam, 550-749, Korea

    Mahinda Senevirathne, Chang-Bum Ahn & Jae-Young Je

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  1. Mahinda Senevirathne
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  2. Chang-Bum Ahn
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  3. Jae-Young Je
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Correspondence to Jae-Young Je.

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Senevirathne, M., Ahn, CB. & Je, JY. Enzymatic extracts from edible red algae, Porphyra tenera, and their antioxidant, anti-acetylcholinesterase, and anti-inflammatory activities. Food Sci Biotechnol 19, 1551–1557 (2010). https://doi.org/10.1007/s10068-010-0220-x

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  • DOI: https://doi.org/10.1007/s10068-010-0220-x

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