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Kosmotoga pacifica sp. nov., a thermophilic chemoorganoheterotrophic bacterium isolated from an East Pacific hydrothermal sediment

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Abstract

A novel strictly anaerobic thermophilic heterotrophic bacterium, strain SLHLJ1T, was isolated from a Pacific hydrothermal sediment. Cells were Gram-negative coccobacilli (approximately 1.0 × 0.6 μm) with a toga. It grew at temperatures between 33 and 78 °C (optimum 70 °C). Elemental sulphur and l-cystine stimulated its growth. It contained C16:0, C16:1 ω11c, C18:0 and C18:1 ω9c as major fatty acids (>5 %), 3 phospholipids and 2 glycolipids as polar lipids. Its DNA G+C content was 43.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLHLJ1T within the family Thermotogaceae. The novel isolate was most closely related to Kosmotoga arenicorallina (97.93 % 16S rRNA gene sequence similarity), K. olearia (92.43 %) and K. shengliensis (92.17 %). On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons with its closest relatives, we propose its assignment to a novel species of the genus Kosmotoga. The name Kosmotoga pacifica sp. nov. is proposed with strain SLHLJ1T (=DSM 26965T = JCM 19180T = UBOCC 3254T) as the type species.

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Acknowledgments

We acknowledge Dr. J.P. Euzéby for support in the Latin etymology of the species name and anonymous reviewers for constructive comments. The Joint Research Unit UMR 6197 (CNRS-Ifremer-UBO), EU program KBBE.2012.3.2-02: Improved cultivation efficiency of marine microorganisms MaCuMBA (Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications), a partenariat Hubert Curien PHC XU GUANGQI collaboration grant n° 27941TL by Egide to MJ, International Sci & Tech Cooperation Program of China (2010DFB23320), National Program on Key Basic Research Project (973 Program, No. 2012CB417300), National Natural Science Foundation of China (No. 41106150) and COMRA project (No. DY125-15-R-01) supported analyses.

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Authors and Affiliations

  1. Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM), UMR 6197, Place Nicolas Copernic, 29280, Plouzané, France

    Stéphane L’Haridon, Karine Alain, Morgane Chalopin, Ouafae Rouxel, Mickaël Beauverger & Mohamed Jebbar

  2. Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), CNRS, IUEM, UMR 6197, Place Nicolas Copernic, 29280, Plouzané, France

    Stéphane L’Haridon, Karine Alain, Morgane Chalopin, Ouafae Rouxel, Mickaël Beauverger & Mohamed Jebbar

  3. Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Technopôle Pointe du diable, Ifremer, UMR 6197, 29280, Plouzané, France

    Stéphane L’Haridon, Karine Alain, Morgane Chalopin, Ouafae Rouxel, Mickaël Beauverger & Mohamed Jebbar

  4. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Daxue Road 184, Xiamen, 361005, China

    Lijing Jiang, Hongxiu Xu & Zongze Shao

Authors
  1. Stéphane L’Haridon
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  2. Lijing Jiang
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  3. Karine Alain
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  4. Morgane Chalopin
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  5. Ouafae Rouxel
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  6. Mickaël Beauverger
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  7. Hongxiu Xu
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  8. Zongze Shao
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  9. Mohamed Jebbar
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Corresponding authors

Correspondence to Karine Alain or Zongze Shao.

Additional information

Communicated by F. Robb.

S. L’Haridon and L. Jiang contributed equally to this work.

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L’Haridon, S., Jiang, L., Alain, K. et al. Kosmotoga pacifica sp. nov., a thermophilic chemoorganoheterotrophic bacterium isolated from an East Pacific hydrothermal sediment. Extremophiles 18, 81–88 (2014). https://doi.org/10.1007/s00792-013-0596-7

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  • DOI: https://doi.org/10.1007/s00792-013-0596-7

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