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Desulfomicrobium thermophilum sp. nov., a novel thermophilic sulphate-reducing bacterium isolated from a terrestrial hot spring in Colombia

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Abstract

A moderately thermophilic, sulphate-reducing bacterium, designated strain P6-2T, was isolated from a terrestrial hot spring located at a height of 2,500 m in the Andean region, Colombia (5°43′69″N, 73°6′10″W). Cells of strain P6-2T were rod-shaped, stained Gram-negative and were motile by means of a single polar flagellum. The strain grew lithotrophically with H2 as the electron donor and organotrophically on lactate, pyruvate, ethanol, malate, fumarate, n-propanol and succinate in the presence of sulphate as the terminal electron acceptor. Fumarate and pyruvate was fermented. Strain P6-2T grew optimally at 55°C (range 37–60°C), pH 6.6 (range 5.8–8.8) in the presence of 0.5% NaCl (range 0–4.5%) with lactate and sulphate and produced acetate, CO2 and H2S as the major end-products. Sulphate, sulphite and thiosulphate could be used as electron acceptors but not elemental sulphur or nitrate. The G + C content of the genomic DNA was 58.7 mol%. The 16S rRNA sequence analysis indicated that strain P6-2T was a member of the class Deltaproteobacteria, domain Bacteria with Desulfomicrobium baculatum being the closest relative (similarity value of 94%). Phylogeny of genes encoding α- and β-subunits of the dissimilatory sulphite reductase (dsrAB genes) supported its affiliation to members of the genus Desulfomicrobium. On the basis of this evidence, we propose to assign strain P6-2T as new species of the genus Desulfomicrobium, D. thermophilum sp. nov., with strain P6-2T as the type strain (= DSM 16697T = CCUG 49732T).

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Acknowledgments

This work was supported by grants from IFS (International Foundation for Sciences), Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología (Colciencias) and Programa Ecos-Nord.

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Authors and Affiliations

  1. IRD, UMR 180 Microbiologie et Biotechnologie des Environnements Chauds, IFR-BAIM, ESIL, Universités de Provence et de la Mediterranée, Case 925, 13288, Marseille, France

    France Thevenieau, Marie-Laure Fardeau, Bernard Ollivier & Catherine Joulian

  2. Environment and Process Division, Biotechnology Unit, BRGM, BP 36009, 45060, Orleans, France

    Catherine Joulian

  3. Unidad de Saneamiento y Biotecnología Ambiental, Departamento de Biología, Pontificia Universidad Javeriana, P.O.B. 56710, Bogota, Colombia

    Sandra Baena

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  1. France Thevenieau
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  2. Marie-Laure Fardeau
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  4. Catherine Joulian
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  5. Sandra Baena
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Correspondence to Sandra Baena.

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Communicated by K. Horikoshi.

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Thevenieau, F., Fardeau, ML., Ollivier, B. et al. Desulfomicrobium thermophilum sp. nov., a novel thermophilic sulphate-reducing bacterium isolated from a terrestrial hot spring in Colombia. Extremophiles 11, 295–303 (2007). https://doi.org/10.1007/s00792-006-0039-9

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