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Evaluation of salivary mucins in children with deciduous and mixed dentition: comparative analysis between high and low caries-risk groups

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Abstract

Objectives

The aim of this study was to examine levels of salivary mucins in children with deciduous and mixed dentition and to determine correlations between salivary mucins and dental caries status in two dentition stages.

Materials and methods

Saliva samples were collected from preschool children with deciduous dentition aged between 4 and 6 years (n = 60) and school children with mixed dentition aged between 9 and 11 years (n = 60). In each age group, the subjects were divided into two categories: high and low caries risk (n = 30 each). Salivary mucins (MUC5B and MUC7) were measured by enzyme-linked immunosorbent assay (ELISA).

Results

There were no significant differences in MUC5B and MUC7 levels between high and low caries-risk groups in preschool children. Significantly increased MUC5B (p = 0.01) and decreased MUC7 (p = 0.04) levels in a low caries-risk group were demonstrated in school children. No significant correlations were observed between salivary mucins and dental caries in preschool children, whereas a significantly negative correlation (r = −0.29, p = 0.03) between MUC5B and the number of decayed teeth was observed in school children.

Conclusion

Patterns of salivary mucin expression in relation to dental caries were different between preschool and school children. The present findings suggest that changes in oral environment from deciduous to mixed dentition may affect the secretion of salivary mucins in response to dental caries.

Clinical relevance

The present study provides additional information that changes in oral environment from deciduous to mixed dentition stage possibly affect the secretion of salivary mucins in response to dental caries.

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Acknowledgments

The authors would like to thank all children and their parents for participation in the study. We also thank teachers in Khon Kaen University’s Demonstration School for their assistance. We would like to thank Dr. Jan GM Bolscher and Dr. Enno CI Veerman for providing primary antibodies against MUC5B and MUC7. This work was supported by a research grant from Khon Kaen University, Thailand. JGMB and ECIV are supported by a grant from the University of Amsterdam for research into the focal point “Oral infections and inflammation.”

Conflict of interest

The authors declare that they have no conflicts of interest.

Author information

Authors and Affiliations

  1. Department of Pediatric Dentistry, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand

    Onauma Angwaravong

  2. Department of Community Dentistry, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand

    Waranuch Pitiphat

  3. Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, 1081 BT, Amsterdam, The Netherlands

    Jan G. M. Bolscher

  4. Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand

    Ponlatham Chaiyarit

  5. Research Group of Chronic Inflammatory Oral Diseases and Systemic Diseases Associated with Oral Health, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand

    Ponlatham Chaiyarit

Authors
  1. Onauma Angwaravong
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  2. Waranuch Pitiphat
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  3. Jan G. M. Bolscher
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  4. Ponlatham Chaiyarit
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Correspondence to Ponlatham Chaiyarit.

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Angwaravong, O., Pitiphat, W., Bolscher, J.G.M. et al. Evaluation of salivary mucins in children with deciduous and mixed dentition: comparative analysis between high and low caries-risk groups. Clin Oral Invest 19, 1931–1937 (2015). https://doi.org/10.1007/s00784-015-1428-1

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  • DOI: https://doi.org/10.1007/s00784-015-1428-1

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