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Taurine supplementation prevents morpho-physiological alterations in high-fat diet mice pancreatic β-cells

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Abstract

Taurine (Tau) is involved in beta (β)-cell function and insulin action regulation. Here, we verified the possible preventive effect of Tau in high-fat diet (HFD)-induced obesity and glucose intolerance and in the disruption of pancreatic β-cell morpho-physiology. Weaning Swiss mice were distributed into four groups: mice fed on HFD diet (36 % of saturated fat, HFD group); HTAU, mice fed on HFD diet and supplemented with 5 % Tau; control (CTL); and CTAU. After 19 weeks of diet and Tau treatments, glucose tolerance, insulin sensitivity and islet morpho-physiology were evaluated. HFD mice presented higher body weight and fat depots, and were hyperglycemic, hyperinsulinemic, glucose intolerant and insulin resistant. Their pancreatic islets secreted high levels of insulin in the presence of increasing glucose concentrations and 30 mM K+. Tau supplementation improved glucose tolerance and insulin sensitivity with a higher ratio of Akt phosphorylated (pAkt) related to Akt total protein content (pAkt/Akt) following insulin administration in the liver without altering body weight and fat deposition in HTAU mice. Isolated islets from HTAU mice released insulin similarly to CTL islets. HFD intake induced islet hypertrophy, increased β-cell/islet area and islet and β-cell mass content in the pancreas. Tau prevented islet and β-cell/islet area, and islet and β-cell mass alterations induced by HFD. The total insulin content in HFD islets was higher than that of CTL islets, and was not altered in HTAU islets. In conclusion, for the first time, we showed that Tau enhances liver Akt activation and prevents β-cell compensatory morpho-functional adaptations induced by HFD.

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Acknowledgments

This study was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ E-26/111.914/2011) and Conselho Nacional para o Desenvolvimento Científico e Tecnológico (CNPq). We are grateful to LD Teixeira for animal care, E.C. Vanzela and J.C. Souza for technical assistance, T.M. Batista for help in data analysis and interpretation and Nicola Conran for editing the English. We also thank E.M. Risso and Prof. Dr. J.A. Farfán from the Laboratório de Fontes Protéicas (Faculdade de Engenharia de Alimentos, UNICAMP) for plasma Tau concentration determination.

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  1. Departamento de Biologia Celular e Estrutural, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil

    Rosane Aparecida Ribeiro, Junia Carolina Santos-Silva, Jean Francisco Vettorazzi, Beatriz Borghi Cotrim, Daniela D. M. Mobiolli, Antonio Carlos Boschero & Everardo Magalhães Carneiro

  2. Laboratório Integrado de Bioquímica Dr. Hatisaburo Masuda, Núcleo em Ecologia e Desenvolvimento Sócio-ambiental de Macaé (NUPEM), Universidade Federal do Rio de Janeiro (UFRJ), Campus Macaé, Macaé, RJ, Brazil

    Rosane Aparecida Ribeiro

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  1. Rosane Aparecida Ribeiro
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  2. Junia Carolina Santos-Silva
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Correspondence to Rosane Aparecida Ribeiro.

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Ribeiro, R.A., Santos-Silva, J.C., Vettorazzi, J.F. et al. Taurine supplementation prevents morpho-physiological alterations in high-fat diet mice pancreatic β-cells. Amino Acids 43, 1791–1801 (2012). https://doi.org/10.1007/s00726-012-1263-5

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  • DOI: https://doi.org/10.1007/s00726-012-1263-5

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