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Molecular characterization of a novel cytorhabdovirus associated with chrysanthemum yellow dwarf disease

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Abstract

Deep-sequencing analysis of a chrysanthemum plant with yellow dwarf symptoms led to the discovery of a novel putative cytorhabdovirus, here tentatively named "chrysanthemum yellow dwarf associated virus" (CYDaV). Its negative-sense single-stranded RNA genome comprises 14,086 nucleotides and contains eight open reading frames in the order 3′ leader-N-P′-P-P3-M-G-P6-L-5′ trailer. CYDaV shares moderate sequence similarity (< 54.2% nucleotide and 51% amino acid sequence identity) with its cytorhabdovirus counterparts in cognate genes. Phylogenetic analysis showed that CYDaV clustered with strong support with alfalfa dwarf virus, raspberry vein chlorosis virus, and strawberry crinkle virus. These findings suggest that CYDaV should be considered a novel member of the genus Cytorhabdovirus, family Rhabdoviridae.

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Acknowledgments

We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript. This research was supported by the National Key R&D Program of China (2019YFD1001800), Fundamental Research Funds for the Central Universities (XDJK2020D016, XDJK2018AA002), Chongqing Research Program of Basic Research and Frontier Technology (cstc2017jcyjBX0016), Innovation Program for Chongqing′s Overseas Returnees (cx2019013), and Overseas Expertise Introduction Project for Discipline Innovation (111 Project) (B18044).

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Authors and Affiliations

  1. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China

    Qiyan Liu, Jingjing Jin, Liu Yang, Song Zhang & Mengji Cao

  2. State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China

    Qiyan Liu, Jingjing Jin, Liu Yang, Song Zhang & Mengji Cao

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  1. Qiyan Liu
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Correspondence to Mengji Cao.

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Qiyan Liu and Jingjing Jin co-first author.

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Liu, Q., Jin, J., Yang, L. et al. Molecular characterization of a novel cytorhabdovirus associated with chrysanthemum yellow dwarf disease. Arch Virol 166, 1253–1257 (2021). https://doi.org/10.1007/s00705-021-04987-2

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  • DOI: https://doi.org/10.1007/s00705-021-04987-2

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