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Detection and analysis of mycovirus-related RNA viruses from grape powdery mildew fungus Erysiphe necator

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Abstract

The fungus, Erysiphe necator Schw., is an important plant pathogen causing powdery mildew disease in grapevines worldwide. In this study, high-throughput sequencing of double-stranded RNA extracted from the fungal tissue combined with bioinformatics was used to examine mycovirus-related sequences associated with E. necator. The results showed the presence of eight mycovirus-related sequences. Five of these sequences representing three new mycoviruses showed alignment with sequences of viruses classified in the genus Alphapartitivirus in the family Partitiviridae. Another three sequences representing three new mycoviruses showed similarity to classifiable members of the genus Mitovirus in the family Narnaviridae. These mycovirus isolates were named Erysiphe necator partitivirus 1, 2, and 3 (EnPV 1-3) and Erysiphe necator mitovirus 1, 2, and 3 (EnMV 1-3) reflecting their E. necator origin and their phylogenetic affiliation with other mycoviruses.

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Author notes

  1. B. Pandey

    Present address: Department of Plant Pathology, North Dakota State University, 306 Walster Hall, Fargo, ND, 58102, USA

Authors and Affiliations

  1. Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser, WA, 99350, USA

    B. Pandey, R. A. Naidu & G. G. Grove

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  1. B. Pandey
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Correspondence to B. Pandey.

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This study was supported by Washington State University Agricultural Research Center.

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First author B. Pandey declares he has no conflict of interest. Second author Naidu A. Rayapati declares he has no conflict of interest Third author Gary G. Grove declares he has no conflict of interest

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Robert H.A. Coutts.

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Pandey, B., Naidu, R.A. & Grove, G.G. Detection and analysis of mycovirus-related RNA viruses from grape powdery mildew fungus Erysiphe necator. Arch Virol 163, 1019–1030 (2018). https://doi.org/10.1007/s00705-018-3714-0

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  • DOI: https://doi.org/10.1007/s00705-018-3714-0

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