Abstract
The complete genome sequences of two newly identified genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) are described. Two isolates, GH11 (18671 nt) and GH30 (18576 nt) were sequenced and found to be less than 70 % similar to other South African GLRaV-3 variants. The genome organization of GH11 and GH30 is similar to that of previously described GLRaV-3 isolates. However, no corresponding open reading frame 2 (ORF2) could be identified. Phylogenetic analysis indicates that GH11 and GH30 cluster in a subgroup (group VI) that is separate from the previously described GLRaV-3 isolates and should be regarded as a different strain of GLRaV-3.
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The financial assistance of the National Research Foundation (NRF) towards this research is hereby acknowledged. Opinions expressed and conclusions arrived at are those of the authors and are not necessarily to be attributed to the NRF. The authors thank Winetech for research funding, Yolandi Nel for her contribution to the survey, and Dr. Dirk Stephan for critical reading of this manuscript.
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Bester, R., Maree, H.J. & Burger, J.T. Complete nucleotide sequence of a new strain of grapevine leafroll-associated virus 3 in South Africa. Arch Virol 157, 1815–1819 (2012). https://doi.org/10.1007/s00705-012-1333-8
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DOI: https://doi.org/10.1007/s00705-012-1333-8