Abstract
Begomovirus is the largest genus within the family Geminiviridae and includes economically important plant DNA viruses infecting a broad range of plant species and causing devastating crop diseases, mainly in subtropical and tropical countries. Besides cultivated plants, many weeds act as virus reservoirs. Eight begomovirus isolates from Bolivian weeds were examined using rolling-circle amplification (RCA) and restriction fragment length polymorphism (RFLP). An efficient, novel cloning strategy using limited Sau3A digestion to obtain tandem-repeat inserts allowed the sequencing of the complete genomes. The viruses were classified by phylogenetic analysis as typical bipartite New World begomoviruses. Four of them represented distinct new virus species, for which the names Solanum mosaic Bolivia virus, Sida mosaic Bolivia virus 1, Sida mosaic Bolivia virus 2, and Abutilon mosaic Bolivia virus are proposed. Three were variants of a new strain of Sida micrantha mosaic virus (SimMV), SimMV-rho[BoVi07], SimMV-rho[Bo:CF1:07] and SimMV-rho[Bo:CF2:07], and one was a new variant of a previously described SimMV, SimMV-MGS2:07-Bo.
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Acknowledgments
We like to thank Christina Wege for critically reading the manuscript, Gaus S. A. Lima and Ulrich Kull for help with the taxonomy of host plants, and the members of the ICTV study group for intense discussions. This work was supported by a trilateral ERA-PG/BMBF project (BMBF 0313986).
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Wyant, P.S., Gotthardt, D., Schäfer, B. et al. The genomes of four novel begomoviruses and a new Sida micrantha mosaic virus strain from Bolivian weeds. Arch Virol 156, 347–352 (2011). https://doi.org/10.1007/s00705-010-0876-9
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DOI: https://doi.org/10.1007/s00705-010-0876-9