Skip to main content

Advertisement

SpringerLink
Log in

Molecular identification of human enteroviruses in children with neurological infections from the central region of Argentina

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

In the central area of Argentina, epidemiological and molecular characteristics of human enterovirus infections are still unknown. RT-nested PCR of the highly conserved 5′NCR was used to detect enteroviruses in 168 samples of cerebrospinal fluid from hospitalized patients with suspected infection of the central nervous system (2007–2008), and 13 (7.7%) were positive. Molecular typing was performed by sequencing of the 3′-half VP1 region. Echovirus 30 was the predominant type detected, followed by coxsackie viruses A9 and B4. All echovirus 30 strains of 2007 clustered in lineage H, whereas the echovirus 30 isolate obtained in 2008 was more distantly related, possibly representing a new lineage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Antona D, Leveque N, Chomel JJ, Dubrou S, Levy-Bruhl D, Lina B (2007) Surveillance of enteroviruses in France, 2000–2004. Eur J Clin Microbiol Infect Dis 26:403–412

    Article  CAS  PubMed  Google Scholar 

  2. Cabrerizo M, Echevarria JE, González I, de Miguel T, Trallero G (2008) Molecular epidemiological study of HEV-B enteroviruses involved in the increase of meningitis cases occurred in Spain during 2006. J Med Virol 80:1018–1024

    Article  PubMed  Google Scholar 

  3. Casas I, Tenorio A, Echevarria JM, Klapper PE, Cleator GM (1997) Detection of enteroviral RNA and specific DNA of herpes viruses by multiplex genome amplification. J Virol Methods 66:39–50

    Article  CAS  PubMed  Google Scholar 

  4. Casas I, Palacios GF, Trallero G, Cisterna D, Freire MC, Tenorio A (2001) Molecular characterization of human enteroviruses in clinical samples: comparison between VP2, VP1, and RNA polymerase regions using RT nested PCR assays and direct sequencing of products. J Med Virol 65:138–148

    Article  CAS  PubMed  Google Scholar 

  5. Castro CM, Oliveira DS, Macedo O, Lima MJ, Santana MB, Wanzeller AL, Silveira E, Gomes M de L (2009) Echovirus 30 associated with cases of aseptic meningitis in state of Pará, Northern Brazil. Mem Inst Oswaldo Cruz 104:444–450

    Google Scholar 

  6. Center of Diseases Control (2003) Outbreaks of aseptic meningitis associated with echoviruses 9 and 30 and preliminary surveillance reports on enterovirus activity-United States. MMWR Morb Mortal Wkly Rep 52:761–764

    Google Scholar 

  7. Cisterna DM, Palacios G, Rivero K, Girard D, Lema C, Freire MC (2007) Epidemiology of enterovirus associated with neurologic diseases. Medicina 67:113–119

    PubMed  Google Scholar 

  8. Choi YJ, Park KS, Baek KA, Jung EH, Nam HS, Kim YB, Park JS (2010) Molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in Korea in 2008. J Microbiol Biotechnol 20:643–649

    CAS  PubMed  Google Scholar 

  9. Freire MC, Cisterna DM, Rivero K, Palacios GF, Casas I, Tenório A, Gomez J (2003) Analysis of an outbreak of viral meningitis in the province of Tucuman, Argentina. Rev Panam Salud Publica 13:246–251

    Article  PubMed  Google Scholar 

  10. Grenón SL, Robledo ML, von Specht MH, Cisterna DM, Lema CL, Freire MC (2008) Outbreak of viral meningitis caused by echovirus type 4 in Misiones province. Rev Argent Microbiol 40:41–46

    PubMed  Google Scholar 

  11. Mistchenko AS, Viegas M, Latta MP, Barrero PR (2006) Molecular and epidemiologic analysis of enterovirus B neurological infection in Argentine children. J Clin Virol 37:293–299

    Article  PubMed  Google Scholar 

  12. Mirand A, Archimbaud C, Henquell C, Michel Y, Chambon M, Peigue-Lafeuille H, Bailly JL (2006) Prospective identification of HEV-B enteroviruses during the 2005 outbreak. J Med Virol 78:1624–1634

    Article  CAS  PubMed  Google Scholar 

  13. Mirand A, Henquell C, Archimbaud C, Peigue-Lafeuille H, Bailly JL (2007) Emergence of recent echovirus 30 lineages is marked by serial genetic recombination events. J Gen Virol 88:166–176

    Article  CAS  PubMed  Google Scholar 

  14. Mueller JE, Bessaud M, Huang QS, Martinez LC, Barril PA, Morel V, BalanantJ BocacaoJ, Hewit J, Gessner BD, Delpeyroux F, Nates SV (2009) Environmental poliovirus surveillance during oral poliovirus vaccine and inactivated poliovirus vaccine use in Cordoba province, Argentina. Appl Environ Microbiol 75:1395–1401

    Article  CAS  PubMed  Google Scholar 

  15. Lukashev AN, Ivanova OE, Eremeeva TP, Gmyl LV (2008) Analysis of echovirus 30 isolates from Russia and new independent states revealing frequent recombination and reemergence of ancient lineages. J Clin Microbiol 46:665–670

    Article  PubMed  Google Scholar 

  16. Oberste MS, Maher K, Kilpatrick DR, Flemister MR, Brown BA, Pallansch MA (1999) Typing of human enteroviruses by partial sequencing of VP1. J Clin Microbiol 37:1288–1293

    CAS  PubMed  Google Scholar 

  17. Oberste MS, Maher K, Williams AJ, Dybdahl-Sissoko N, Brown BA, Gookin MS, Peñaranda S, Mishrik N, Uddin M, Pallansch MA (2006) Species-specific RT-PCR amplification of human enteroviruses: a tool for rapid species identification of uncharacterized enteroviruses. J Gen Virol 87:119–128

    Article  CAS  PubMed  Google Scholar 

  18. Palacios G, Casas I, Cisterna D, Trallero G, Tenorio A, Freire C (2002) Molecular epidemiology of echovirus 30: temporal circulation and prevalence of single lineages. J Virol 76:4940–4949

    Article  CAS  PubMed  Google Scholar 

  19. Roth B, Enders M, Arents A, Pfitzner A, Terletskaia-Ladwig E (2007) Epidemiologic aspects and laboratory features of enterovirus infections in Western Germany, 2000–2005. J Med Virol 79:956–962

    Article  PubMed  Google Scholar 

  20. Saeed M, Zaidi SZ, Naeem A, Masroor M, Sharif S, Shaukat S, Angez M, Khan A (2007) Epidemiology and clinical findings associated with enteroviral acute flaccid paralysis in Pakistan. BMC Infect Dis 7:6

    Article  PubMed  Google Scholar 

  21. She RC, Hymas WC, Taggart EW, Petti CA, Hillyard DR (2010) Performance of enterovirus genotyping targeting the VP1 and VP2 regions on non-typeable isolates and patient specimens. J Virol Methods 165:46–50

    Article  CAS  PubMed  Google Scholar 

  22. Stanway G, Brown F, Christian P, Hovi T, Hyypiä T, King AMQ, Knowles NJ, Lemon SM, Minor PD, Pallansch MA, Palmenberg AC, Skern T (2005). Family Picornaviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus Taxonomy. 8th Report of the International Committee on Taxonomy of Viruses. Elsevier/Academic Press, London, pp. 757–778

  23. Thoelen I, Moes E, Lemey P, Mostmans S, Wollants E, Lindberg AM, Vandamme AM, Van Ranst M (2004) Analysis of the serotype and genotype correlation of VP1 and the 50 noncoding region in an epidemiological survey of the human enterovirus B species. J Clin Microbiol 42:963–971

    Article  CAS  PubMed  Google Scholar 

  24. Trallero G, Casas I, Tenorio A, Echevarria JE, Castellanos A, Lozano A, Breña P (2000) Enteroviruses in Spain: virological and epidemiological studies over ten years (1988–1997). Epidemiol Infect 124:497–506

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported in part by grants from SECyT (Secretaría de Ciencias, Tecnología e Innovación Productiva, Argentina). M. Cabrerizo is supported by the Spanish Ministry of Health (DGEG-1304/08). V. Ré is a scientific member of CONICET, Argentina. M.B. Pisano is recipient of the CONICET scholarships of Argentina.

Author information

Authors and Affiliations

  1. Instituto de Virología “Dr. J. M. Vanella”, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Cordoba, Argentina

    Adrián Farías, Viviana Ré, Belén Pisano, Lorena Spinsanti & Marta Silvia Contigiani

  2. Departamento de Epidemiología, Ministerio de Salud de la Provincia de Córdoba, Cordoba, Argentina

    Adrián Farías & Nora Glatstein

  3. National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain

    María Cabrerizo

  4. Enfermera Gordillo Gómez s/n Ciudad Universitaria, CP 5016, Cordoba, Argentina

    Adrián Farías

Authors
  1. Adrián Farías
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. María Cabrerizo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Viviana Ré
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nora Glatstein
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Belén Pisano
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lorena Spinsanti
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Marta Silvia Contigiani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adrián Farías.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Farías, A., Cabrerizo, M., Ré, V. et al. Molecular identification of human enteroviruses in children with neurological infections from the central region of Argentina. Arch Virol 156, 129–133 (2011). https://doi.org/10.1007/s00705-010-0828-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-010-0828-4

Keywords

Search

Navigation