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The effect of human immunodeficiency virus type 1 (HIV-1) gp41 variability on antibody detection

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Abstract

To determine whether genetic variability influences the ability to detect antibody, nine gp41 ectodomain recombinant proteins from human immunodeficiency virus type 1 (HIV-1) CRF07_BC, CRF01_AE and subtype B′ were expressed in a bacterial expression system and purified. An indirect sandwich ELISA was developed with individual purified recombinant proteins. Plasma samples from 26 individuals infected with HIV-1 of different subtypes and four samples from the 1st international antibody reference panel were tested against each recombinant protein by ELISA. Heat-map and two-dimensional hierarchical clustering methods revealed that ELISA reactivity against antigens derived from the same subtypes clustered together. This suggests a similar reactivity pattern among infections of the same subtype, and thus the antigenicity of gp41 recombinant proteins may vary depending on the subtype, and subtype-related serotypes may exist among these antigens. Using association analysis methods, eight signature sites related to the subtype-specific reactivity patterns were identified. This study provides valuable information for the development of diagnostic assays with the ability to detect broadly cross-reactive antibodies induced by infection with different HIV-1 subtypes.

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Acknowledgments

We would like to thank Dr. Zhiqiang He (Fapon Biotech Inc.) for technical assistance with protein expression and Dr. Yi Ye, Feifei Dai, Qifeng Zhang and Zhi Zhang (Kinghawk Pharmaceutical Co., Ltd) for technical assistance with ELISA assays. This study was supported by the key project on infectious diseases such as AIDS, Hepatitis, Tuberculosis (grant: 2009ZX10004-801) from the Ministry of Science and Technology, China.

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Authors and Affiliations

  1. Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, No. 2 Tiantanxili, 100050, Beijing, People’s Republic of China

    Jianhui Nie, Aijing Song, Sihong Xu, Xiuhua Li & Youchun Wang

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  1. Jianhui Nie
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  2. Aijing Song
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  3. Sihong Xu
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  4. Xiuhua Li
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Correspondence to Youchun Wang.

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Nie, J., Song, A., Xu, S. et al. The effect of human immunodeficiency virus type 1 (HIV-1) gp41 variability on antibody detection. Arch Virol 155, 1813–1822 (2010). https://doi.org/10.1007/s00705-010-0775-0

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  • DOI: https://doi.org/10.1007/s00705-010-0775-0

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