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Variation in Saffron (Crocus sativus L.) accessions and Crocus wild species by RAPD analysis

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Abstract

Crocus, a genus of Iridaceae is mostly grown in areas with Mediterranean environment as the best region. Saffron (C. sativus) is a perennial plant and is cultivated as an industrial crop in several regions of Iran. In this research, five accessions of cultivated Saffron from five areas in Khorasan, and Esfahan including Gonabad, Ferdows, Torbat-e-Heyidariye, Estahbanat, Gopayegan were used. Other nine species of saffron were grown naturally in Iran; so we collected two wild species (C. caspius and C. speciosus) from north of Iran (Gilan Province). RAPD markers were used to classify these species and to find their relationships. In the results of this study, the cluster analysis showed two distinct groups. Also, the maximum similarity was seen between C. caspius and C. speciosus (0.82) and the minimum was between Estahbanat, Ferdows accessions and C. speciosus (0.33). Finally, this method as a convenience procedure could be used to separate different accessions and species of Crocus as well.

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References

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation version II. Plant Mol Biol Report 1:19–21

    Article  CAS  Google Scholar 

  • Grilli Caiola M, Caputo P, Zanier R (2004) RAPD analysis in Crocus sativus L. accessions and related Crocus species. Biol Plant 48:375–380

    Article  Google Scholar 

  • Lage M, Cantrell CL (2009) Quantification of saffron (Crocus sativus L.) metabolites crocins, picrocrocin and safranal for quality determination of the spice grown under different environmental Moroccan conditions. Sci Hortic 121:366–373

    Article  CAS  Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Rashed-Mohassel MH (2007) Saffron from wild to the field. Acta Hort (ISHS) 739:187–193

    Google Scholar 

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Acknowledgments

The authors wish to thank Dr. Mardi in ABRII institute for his cooperation in preparing materials.

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Correspondence to F. Izadpanah.

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Izadpanah, F., Kalantari, S., Hassani, M.E. et al. Variation in Saffron (Crocus sativus L.) accessions and Crocus wild species by RAPD analysis. Plant Syst Evol 300, 1941–1944 (2014). https://doi.org/10.1007/s00606-014-1018-8

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  • DOI: https://doi.org/10.1007/s00606-014-1018-8

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