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Laser-induced graphene electrodes on polyimide membranes modified with gold nanoparticles for the simultaneous detection of dopamine and uric acid in human serum

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Abstract

The level control of biological active molecules in human body fluids is important for the surveillance of several human diseases. Dopamine (DA) and uric acid (UA) are two important biomarkers of neurological and bone diseases, respectively. Design of sensitive and cost-effective sensors for their detection is an effervescent research field. We report on the straightforward design of laser-induced graphene electrodes (LIGEs) from the laser ablation of a polyimide substrate and their modification by electrochemical deposition of gold nanoparticles (AuNPs/LIGE) and their uses as chemosensors. Electrochemical investigations showed that the presence of gold nanoclusters onto the electrode surface improved the electrochemical surface area (ECSA) and the heterogenous electron transfer (HET) rate. Furthermore, the AuNPs/LIGEs can be used to detect simultaneously low concentrations of DA and UA in presence of ascorbic acid (AA) as an potentially interfering substance at redox potentials of 300 mV, 230 mV and 450 mV and 91 mV, respectively, compared with the Ag/AgCl (3 M KCl) reference electrode in cyclic voltametric. The method displayed linear ranges varying from 2 to 20 μM and 5 to 50 μM, led to limits of detection of 0.37 μM and 0.71 μM for DA and UA, respectively. The AuNPs/LIGE was applied to simultaneously detect both analytes in scarcely diluted human serum with good recoveries. The data show that the recovery percentages ranged from 94% ± 2.1 to 102 % ± 0.5 and from 94% ±0.3 to 112% ± 1.4 for dopamine and uric acid, respectively. Thus, the AuNPs/LIGEs are promising candidates for the detection of other biologically active molecules such as drugs, pesticides, and metabolites.

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Data Availability

Data sets generated during the current study are available from the corresponding author on reasonable request.

Abbreviations

AA:

Ascorbic acid

ASSURED:

Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free and Deliverable to end-users

AuNPs:

Gold Nanoparticles

CV:

Cyclic Voltammetry

DA:

Dopamine

DPV:

Differential Pulse Voltammetry

EIS:

Electrochemical Impedance Spectroscopy

LIGEs:

Laser-Induced Graphene Electrodes

PI:

Polyimide

UA:

Uric acid

UV:

ultraviolet

WHO:

World Health Organization

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Acknowledgements

The authors wish to express their gratitude to the Ministry of Higher Education, Research and Innovation of Burkina Faso through the Higher Education Support Project (PAES) financially supported by World Bank. Ms Bibata OUEDRAOGO would like to thank the International Science Programme (ISP) through the African Network of Electroanalytical Chemists (ANEC) for the scholarship.

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Authors and Affiliations

  1. Université Joseph KI-ZERBO, UFR-SEA, Laboratoire de Chimie Analytique, Environnementale et Biorganique (LCAEBiO), 03 BP 7021, Ouagadougou 03, Burkina Faso

    Bibata Ouedraogo, Amidou Tall & Issa Tapsoba

  2. Université de Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Chimie Analytique et Electrochimie (LR99ES15), Sensors and Biosensors Group, 2092, Tunis, El Manar, Tunisia

    Sabrine Baachaoui & Noureddine Raouafi

  3. Laboratoire de Sciences et Technologies (LaST), Université Thomas SANKARA, 12 BP 417, Ouagadougou, Burkina Faso

    Amidou Tall

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  1. Bibata Ouedraogo
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Correspondence to Noureddine Raouafi.

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Ouedraogo, B., Baachaoui, S., Tall, A. et al. Laser-induced graphene electrodes on polyimide membranes modified with gold nanoparticles for the simultaneous detection of dopamine and uric acid in human serum. Microchim Acta 190, 316 (2023). https://doi.org/10.1007/s00604-023-05909-6

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