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A dual-model strategy for fluorometric determination of ascorbic acid and of ascorbic acid oxidase activity by using DNA-templated gold-silver nanoclusters

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Abstract

Methods are described for the fluorometric and colorimetric determination of ascorbic acid (AA) and the activity of ascorbic acid oxidase (AA-Ox). The method for AA is based on AA-induced growth and aggregation of DNA-templated gold/silver nanoclusters (DNA-Au/Ag NC), which is accompanied by quenching of fluorescence emission at 605 nm upon 260 nm excitation and a visible color change of the solution from colorless transparent to yellow. The determination of the activity of AA-Ox is based on the finding that it catalyzes the oxidation of AA which results in the inhibition of growth and aggregation. AA can be determined with a 0.6 μmol·L−1 detection limit over the 5 to 150 μmol·L−1 concentration range. AA-Ox can be determined with a 0.0048 U·mL−1 detection limit over the 0.01 to 0.20 U·mL−1 range.

Schematic of a novel fluorometric and colorimetric platform for determination of ascorbic acid and ascorbic acid oxidase activity based on the use of DNA-templated gold-silver nanoclusters.

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Acknowledgements

This work was supported by the Fundamental Research Funds for Central Universities of China (N152004002) and the Doctoral Scientific Research Foundation of Liao Ning Province (L201501145).

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Authors and Affiliations

  1. College of Life and Health Sciences, Northeastern University, Shenyang, 110000, China

    Siyu Liu

  2. College of chemistry, chemical engineering and environmental engineering, Liaoning Shihua University, Fushun, 113001, China

    Shu Pang

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  1. Siyu Liu
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Correspondence to Siyu Liu or Shu Pang.

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Liu, S., Pang, S. A dual-model strategy for fluorometric determination of ascorbic acid and of ascorbic acid oxidase activity by using DNA-templated gold-silver nanoclusters. Microchim Acta 185, 426 (2018). https://doi.org/10.1007/s00604-018-2954-8

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  • DOI: https://doi.org/10.1007/s00604-018-2954-8

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