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A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

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Abstract

We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N,N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3-trimethylsilanyl-prop-2-yne methacrylate (MAPS) and specifically adapted to thiol-ene formulations in order to obtain a neutral and permanent coating for microchannels. The performance of two different thiol-ene substrates (with 20 % and 40 % excess of thiol groups, respectively) coated with either p-(DMA-PMA) or p-(DMA-PMA-MAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption. Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most efficient charge shielding in the pH range from 3 to 9. The modified microchannels were successfully applied to electrokinetic separations of acidic and basic proteins.

A thiol-ene microchip coated with a poly(dimethylacrylamide)-based copolymer was developed for microchip electrophoresis applications. Surface charge blocking, protein adsorption elimination and electrophoretic separations of acidic and basic proteins were successfully demonstrated using this polymer coated thiol-ene microchip platform.

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Acknowledgments

This work, the post-doctoral fellowship for Dr. Thanh Duc Mai and the PhD scholarship for Kiarach Mesbah have been financially supported by the European Community’s Seventh Framework Programme (NaDiNe FP7/2010-2015) under the grant agreement n° 246513. We would like to thank Nacéra Aboud (PNAS, Université Paris Sud) for valuable discussion and Andreas Hjarne Kunding (Technical University of Denmark) for his assistance with chip fabrication.

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Authors and Affiliations

  1. Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, 5 rue JB Clément, 92296, Châtenay-Malabry, France

    Kiarach Mesbah, Thanh Duc Mai & Myriam Taverna

  2. Department of Micro and Nanotechnology, Technical University of Denmark, DTU, 2800, Kongens Lyngby, Denmark

    Thomas Glasdam Jensen

  3. Istituto di Chimica del Riconoscimento Molecolare (ICRM) - C.N.R., Via Mario Bianco, 9, 20131, Milano, Italy

    Laura Sola & Marcella Chiari

  4. Department of Pharmacy, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark

    Jörg P. Kutter

Authors
  1. Kiarach Mesbah
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  2. Thanh Duc Mai
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  3. Thomas Glasdam Jensen
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  4. Laura Sola
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  5. Marcella Chiari
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  6. Jörg P. Kutter
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  7. Myriam Taverna
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Correspondence to Myriam Taverna.

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Mesbah, K., Mai, T.D., Jensen, T.G. et al. A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis. Microchim Acta 183, 2111–2121 (2016). https://doi.org/10.1007/s00604-016-1825-4

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  • DOI: https://doi.org/10.1007/s00604-016-1825-4

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