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Highly sensitive and selective electrochemical detection of L-cysteine using nanoporous gold

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Abstract

Nanoporous gold (NPG) with uniform pore sizes and ligaments was prepared by using a simple dealloying method. NPG electrodes exhibit excellent electrocatalytic activity towards the oxidation of CySH and the mechanism for the electrochemical reaction of CySH on NPG has been discussed. Interestingly, if the operating potential is fixed at 0.65 V, a strong current is observed and interferences by tryptophan and tyrosine are avoided. The calibration plot is linear in the concentration range from 1 μM to 400 μM (R2 = 0.994), and the quantification limit is as low as 50 nM. The NPG-modified electrode has good reproducibility, high sensitivity and selectivity, can be used to sense CySH in aqueous solution.

Nanoporous gold with uniform pore sizes and ligaments was employed as sensitive and selective electrochemical sensor for detection of Lcysteine, and the quantification limit is as low as 50 nM.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (21175059, 21073111), Graduate Independent Innovation Foundation of Shandong University, and National Basic Research Program of China (2009CB93103).

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Authors and Affiliations

  1. Key Laboratory for Colloid and Interface Chemistry of State Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China

    Zhaona Liu, Huacheng Zhang & Houyi Ma

  2. Department of Chemistry and Biochemistry, Montclair State University, Montclair, NJ, 07043, USA

    Shifeng Hou

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  1. Zhaona Liu
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  2. Huacheng Zhang
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  3. Shifeng Hou
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  4. Houyi Ma
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Correspondence to Houyi Ma.

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Liu, Z., Zhang, H., Hou, S. et al. Highly sensitive and selective electrochemical detection of L-cysteine using nanoporous gold. Microchim Acta 177, 427–433 (2012). https://doi.org/10.1007/s00604-012-0801-x

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  • DOI: https://doi.org/10.1007/s00604-012-0801-x

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