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The physiological and nutritional responses to an excess of boron by Verna lemon trees that were grafted on four contrasting rootstocks

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Abstract

Citrus species are sensitive to an excess of boron (B). Currently, this toxicity is becoming a serious problem in the soils of arid and semi-arid environments throughout the world, where high concentrations of B may occur due to the agricultural use of wastewater. Citrus rootstocks can greatly influence the tolerance of citrus trees to different abiotic stresses. However, little is known about how the rootstock influences the tolerance of these trees to an excess of B. In this study, the effects of the nutrient solution’s B concentration (0.25, 2, 4.5 or 7 mg l−1) on the growth and other physiological, nutritional and biochemical parameters of Verna lemon trees that were grafted on four contrasting rootstocks [Carrizo citrange (CC), Cleopatra mandarin (CL), Citrus macrophylla (CM) and sour orange (SO)] were investigated. The plants were grown in a greenhouse in pots containing a universal substrate media and were watered daily with a Hoagland nutrient solution containing different concentrations of B. The results showed that the plant growth was progressively inhibited with an increasing concentration of B in the nutrient solution. However, the shoot was more sensitive to the B toxicity than were the roots. In addition, the growth inhibition was reduced in trees that were grafted on CL and CM when compared with those that were grafted on CC and SO. The concentration of B in the leaves, stems and roots also increased with an increase in the concentration of external B in the following order: leaves > roots > stem. The rootstock also had an influence on the B concentration in the different plant tissues. In the leaves, the B concentration was lowest in the plants that were grafted on the SO rootstock followed by the plants that were grafted on either the CM or CL rootstock and highest in the plants that were grafted on the CC rootstock. The net assimilation of CO2 (\( A_{{{\text{CO}}_{2} }} \)) and the stomatal conductance (g s) leaf gas exchange parameters were reduced with an excess of B in the leaves, and this reduction was less pronounced for trees on CM and CL. The intercellular CO2 concentration (C i) and the chlorophyll fluorescence indicated that the reduction of \( A_{{{\text{CO}}_{2} }} \) that was found with an excess of B was mainly due to non-stomatal factors. The mineral nutrition and organic solute data are also shown in this study. All of the data indicate that the tolerance to an excess of B is not related to the concentration of B that has accumulated in the leaves, which indicates that a combination of rootstock-dependent physiological, biochemical and anatomical responses determine the tolerance to an excess of B in citrus plants.

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Acknowledgments

V. Gimeno is a Ph.D. student supported by the Fundación Seneca (Región de Murcia). Funding for this research came from the Ministry of Education and Science, Government of Spain (Project Plan National AGL2007-65437-C04-02/AGR; AGL2011-24795).

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Authors and Affiliations

  1. Centro de Edafología y Biología Aplicada del Segura, CSIC, Campus Universitario de Espinardo, 30100, Espinardo, (Murcia), Spain

    Vicente Gimeno, Vicente Martínez & Francisco García-Sánchez

  2. EPSO (Univ. Miguel Hernández), Ctra. Beniel km 3,2, 03312, Orihuela, (Alicante), Spain

    Inma Simón, Manuel Nieves & José M. Cámara-Zapata

  3. Facultad de Química, Universidad de Murcia.Campus Universitario de Espinardo, 30071, Espinardo, (Murcia), Spain

    Antonio L. García

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  1. Vicente Gimeno
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  2. Inma Simón
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  3. Manuel Nieves
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  4. Vicente Martínez
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  7. Francisco García-Sánchez
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Correspondence to Vicente Gimeno.

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Communicated by T. Koike.

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Gimeno, V., Simón, I., Nieves, M. et al. The physiological and nutritional responses to an excess of boron by Verna lemon trees that were grafted on four contrasting rootstocks. Trees 26, 1513–1526 (2012). https://doi.org/10.1007/s00468-012-0724-5

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