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Development of bisphenol A-removing recombinant Escherichia coli by monomeric and dimeric surface display of bisphenol A-binding peptide

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Abstract

Peptide-displaying Escherichia coli cells were investigated for use in adsorptive removal of bisphenol A (BPA) both in Luria–Bertani medium including BPA or ATM thermal paper eluted wastewater. Two recombinant strains were constructed with monomeric and dimeric repeats of the 7-mer BPA-binding peptide (KSLENSY), respectively. Greater than threefold increased adsorption of BPA [230.4 µmol BPA per g dry cell weight (DCW)] was found in dimeric peptide-displaying cells compared to monomeric strains (63.4 µmol per g DCW) in 15 ppm BPA solution. The selective removal of BPA from a mixture of BPA analogs (bisphenol F and bisphenol S) was verified in both monomeric and dimeric peptide-displaying cells. The binding chemistry of BPA with the peptide was assumed, based on molecular docking analysis, to be the interaction of BPA with serine and asparagine residues within the 7-mer peptide sequence. The peptide-displaying cells also functioned efficiently in thermal paper eluted wastewater containing 14.5 ppm BPA.

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References

  1. Michałowicz J (2014) Bisphenol A—sources, toxicity and biotransformation. Environ Toxicol Pharmacol 37:738–758

    Article  Google Scholar 

  2. Rajasärkkä J, Koponen J, Airaksinen R, Kiviranta H, Virta M (2014) Monitoring bisphenol A and estrogenic chemicals in thermal paper with yeast-based bioreporter assay. Anal Bioanal Chem 406:5695–5702

    Article  Google Scholar 

  3. Liao C, Kannan K (2011) Widespread occurrence of bisphenol A in paper and paper products: implications for human exposure. Environ Sci Technol 45:9372–9379

    Article  CAS  Google Scholar 

  4. Geens T, Goeyens L, Kannan K, Neels H, Covaci A (2012) Levels of bisphenol-A in thermal paper receipts from Belgium and estimation of human exposure. Sci Total Environ 435–436:30–33

    Article  Google Scholar 

  5. Porras SP, Heinälä M, Santonen T (2014) Bisphenol A exposure via thermal paper receipts. Toxicol Lett 230:413–420

    Article  CAS  Google Scholar 

  6. Mendum T, Stoler E, VanBenschoten H, Warner JC (2010) Concentration of bisphenol A in thermal paper. Green Chem Lett Rev 4:81–86

    Article  Google Scholar 

  7. Bae W, Wu CH, Kostal J, Mulchandani A, Chen W (2003) Enhanced mercury biosorption by bacterial cells with surface-displayed MerR. Appl Environ Microbio 69:3176–3180

    Article  CAS  Google Scholar 

  8. Li P-S, Tao H-C (2013) Cell surface engineering of microorganisms towards adsorption of heavy metals. Crit Rev Microbio 41:140–149

    Article  Google Scholar 

  9. Cruz N, Le Borgne S, Herna´ndez-Cha´vez G, Gosset G, Valle F, Bolivar F (2000) Engineering the Escherichia coli outer membrane protein OmpC for metal bioadsorption. Biotechnol Lett 22:623–629

    Article  CAS  Google Scholar 

  10. Maruthamuthu M, Nadarajan S, Ganesh I, Ravikumar S, Yun H, Yoo I-k, Hong S (2015) Construction of a high efficiency copper adsorption bacterial system via peptide display and its application on copper dye polluted wastewater. Bioproc Biosyst Eng 38:2077–2084

    Article  CAS  Google Scholar 

  11. Ravikumar S, Ganesh I, Yoo I-K, Hong SH (2012) Construction of a bacterial biosensor for zinc and copper and its application to the development of multifunctional heavy metal adsorption bacteria. Process Biochem 47:758–765

    Article  CAS  Google Scholar 

  12. Maruthamuthu M, Ganesh I, Ravikumar S, Hong S (2015) Evaluation of zraP gene expression characteristics and construction of a lead (Pb) sensing and removal system in a recombinant Escherichia coli. Biotechnol Lett 37:659–664

    Article  CAS  Google Scholar 

  13. Ravikumar S, Yoo I-K, Lee SY, Hong SH (2011) A study on the dynamics of the zraP gene expression profile and its application to the construction of zinc adsorption bacteria. Bioproc Biosyst Eng 34:1119–1126

    Article  CAS  Google Scholar 

  14. Yoo I-K, Choe WS (2013) Screening of peptide sequences with affinity to bisphenol A by biopanning. Korean J Microbiol 49:211–214

    Article  Google Scholar 

  15. Baslé A, Rummel G, Storici P, Rosenbusch JP, Schirmer T (2006) Crystal structure of osmoporin OmpC from E. coli at 2.0 Å. J Mol Biol 362:933–942

    Article  Google Scholar 

  16. Eswar N, Webb B, Marti-Renom MA, Madhusudhan MS, Eramian D, Shen M-Y, Pieper U, Sali A (2001) Comparative protein structure modeling using MODELLER. Current protocols in protein science. Wiley, New Jersey

    Google Scholar 

  17. Laskowsky RAMM., Moss DS, Thornton JM (1993) PROCHECK: a program to check the stereo chemical quality of protein structures. J Appl Crystallogr 26:283–291

    Article  Google Scholar 

  18. Ramachandran GNRC., Sasisekharan V (1963) Stereochemistry of polypeptide chain configurations. J Mol Biol 7:95–99

    Article  CAS  Google Scholar 

  19. Bhattacharya D, Cheng J (2013) 3Drefine: consistent protein structure refinement by optimizing hydrogen bonding network and atomic-level energy minimization. Proteins 81:119–131

    Article  CAS  Google Scholar 

  20. Wiederstein M, Sippl MJ (2007) ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins. Nucleic Acids Res 35:W407-W410

    Article  Google Scholar 

  21. Patra SM, Baştuǧ T, Kuyucak S (2007) Binding of organic cations to gramicidin A channel studied with autodock and molecular dynamics simulations. J Phys Chem B 111:11303–11311

    Article  CAS  Google Scholar 

  22. Salentin S, Schreiber S, Haupt VJ, Adasme MF, Schroeder M (2015) PLIP: fully automated protein–ligand interaction profiler. Nucleic Acids Res 43:W443-W447

    Article  Google Scholar 

  23. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nat 227:680–685

    Article  CAS  Google Scholar 

  24. Han W, Luo L, Zhang S (2012) Adsorption of bisphenol A on lignin: effects of solution chemistry. Int J Environ Sci Technol 9:543–548

    Article  CAS  Google Scholar 

  25. Lazim ZM, Hadibarata T, Puteh MH, Yusop Z (2015) Adsorption characteristics of bisphenol A onto low-cost modified phyto-waste material in aqueous solution. Water Air Soil Poll 226:34

    Article  Google Scholar 

  26. Endo Y, Kimura N, Ikeda I, Fujimoto K, Kimoto H (2007) Adsorption of bisphenol A by lactic acid bacteria, Lactococcus, strains. Appl Microbiol Biotechnol 74:202–207

    Article  CAS  Google Scholar 

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2013R1A1A2004799).

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Authors and Affiliations

  1. School of Chemical Engineering, University of Ulsan, Ulsan, 680-749, Republic of Korea

    Murali kannan Maruthamuthu, Jiyeon Hong, Sivachandiran Somasundaram, SoonHo Hong & Ik-Keun Yoo

  2. Department of Biotechnology, Indian Institute of Technology Madras, Chennai, Tamil nadu, 600036, India

    Kulandaisamy Arulsamy

  3. School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Republic of Korea

    Woo-Seok Choe

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  1. Murali kannan Maruthamuthu
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  2. Jiyeon Hong
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  3. Kulandaisamy Arulsamy
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  4. Sivachandiran Somasundaram
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  5. SoonHo Hong
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  6. Woo-Seok Choe
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  7. Ik-Keun Yoo
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Correspondence to Ik-Keun Yoo.

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Maruthamuthu, M.k., Hong, J., Arulsamy, K. et al. Development of bisphenol A-removing recombinant Escherichia coli by monomeric and dimeric surface display of bisphenol A-binding peptide. Bioprocess Biosyst Eng 41, 479–487 (2018). https://doi.org/10.1007/s00449-017-1882-z

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  • DOI: https://doi.org/10.1007/s00449-017-1882-z

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